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产品描述：人Rho GDP解离抑制因子2(ARHGDIB)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的ARHGDIB含量。试剂盒检测范围为25 pg/mL-1600 pg/mL，适用于基础研究中ARHGDIB蛋白表达水平的定量分析，例如细胞信号转导机制探索、疾病模型构建或药物干预效果评估等科研场景本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Arhgdib ELISA Kit; D4 ELISA Kit; D4 GDP dissociation inhibitor ELISA Kit; GDIA 2 ELISA Kit; GDIA2 ELISA Kit; GDID 4 ELISA Kit; GDID4 ELISA Kit; GDIR2_HUMAN ELISA Kit; GDP dissociation inhibitor D4 ELISA Kit; LY GDI ELISA Kit; Ly-GDI ELISA Kit; LYGDI ELISA Kit; MGC108926 ELISA Kit; RAP1GN1 ELISA Kit; Rho GDI 2 ELISA Kit; Rho GDI beta ELISA Kit; Rho GDI2 ELISA Kit; Rho GDP dissociation inhibitor (GDI) beta ELISA Kit; Rho GDP dissociation inhibitor 2 ELISA Kit; Rho GDP dissociation inhibitor beta ELISA Kit; Rho GDP-dissociation inhibitor 2 ELISA Kit; Rho-GDI beta ELISA Kit; RhoGDI2 ELISA Kit
缩写：ARHGDIB
Uniprot No.：P52566
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：25 pg/mL-1600 pg/mL
灵敏度：6.25 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human ARHGDIB in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 87 | |||||
| Range % | 85-93 | ||||||
| 1:2 | Average % | 104 | |||||
| Range % | 96-108 | ||||||
| 1:4 | Average % | 92 | |||||
| Range % | 85-96 | ||||||
| 1:8 | Average % | 101 | |||||
| Range % | 98-105 | ||||||
回收率：
| The recovery of human ARHGDIB spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 92 | 88-96 | |||||
| EDTA plasma (n=4) | 103 | 98-107 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| pg/mlOD1OD2AverageCorrected 16002.9212.8442.8832.711 8002.3342.2412.2882.116 4001.5341.4911.5131.341 2000.8870.8670.8770.705 1000.5960.5810.5890.417 500.3700.3620.3660.194 250.2380.2340.2360.064 00.1730.1710.172 | |||||||
货期：3-5 working days
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