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产品描述：人精氨酸酶2(ARG2)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、尿液、脑脊液（CSF）样本中的ARG2含量。ARG2即精氨酸酶 2，是一种含锰金属酶。它在多种生理和病理过程中发挥作用。研究认为其可调节精氨酸代谢，影响一氧化氮生成。在心血管疾病、炎症性疾病等的发病机制中ARG2参与其中，对其研究有助于揭示疾病机制并为治疗提供新思路。试剂盒检测范围为0.47 mU/mL-30 mU/mL，适用于细胞模型研究、疾病机制探索、生物标志物筛选及药物干预效果评估等非临床研究场景；支持科研人员探究不同生理或病理条件下ARG2的活性变化；为体外实验提供高效可靠的分析工具本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：ARG2 ELISA Kit; ARGI2_HUMAN ELISA Kit; Arginase II mitochondrial ELISA Kit; Arginase type II ELISA Kit; Arginase-2 ELISA Kit; arginase-2, mitochondrial ELISA Kit; Kidney arginase ELISA Kit; Kidney type arginase ELISA Kit; Kidney-type arginase ELISA Kit; L arginine amidinohydrolase ELISA Kit; L arginine ureahydrolase ELISA Kit; mitochondrial ELISA Kit; Non hepatic arginase ELISA Kit; Non-hepatic arginase ELISA Kit; Nonhepatic arginase ELISA Kit; Type II arginase ELISA Kit
缩写：ARG2
Uniprot No.：P78540
种属：Homo sapiens (Human)
样本类型：serum, plasma, cell culture supernates, urine, cerebrospinal fluid (CSF)
检测范围：0.47 mU/mL-30 mU/mL
灵敏度：0.12 mU/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Metabolism
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human ARG2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 87 | ||||
| Range % | 83-91 | |||||
| 1:2 | Average % | 104 | ||||
| Range % | 101-107 | |||||
| 1:4 | Average % | 96 | ||||
| Range % | 92-99 | |||||
| 1:8 | Average % | 85 | ||||
| Range % | 82-89 | |||||
回收率：
| The recovery of human ARG2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 94 | 91-98 | ||||
| EDTA plasma (n=4) | 90 | 86-97 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| mU/mlOD1OD2AverageCorrected302.3272.2982.3132.146151.8201.8011.8111.6447.51.3931.3781.3861.2193.750.9790.9980.9890.8221.870.5640.6100.5870.4200.940.3960.3760.3860.2190.470.2730.2950.2840.11700.1660.1680.167 | ||||||
货期：3-5 working days
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