首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人载脂蛋白M(APOM)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液样本中的APOM含量。APOM是一个靶点。它具有一定的生物学背景，在体内发挥特定作用。目前研究聚焦其在相关生理和病理过程的机制，可能参与脂质代谢、心血管调节等。深入探究其作用机制，有助于为相关疾病的治疗提供新靶点和思路。试剂盒检测范围为1.56 ng/mL-100 ng/mL，该产品适用于基础科研中APOM相关脂代谢机制研究、心血管疾病模型评估、药物干预效果分析等场景，为探索APOM在病理生理过程中的作用提供可靠工具本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Apo M ELISA Kit; Apo-M ELISA Kit; Apolipoprotein M ELISA Kit; ApoM ELISA Kit; APOM_HUMAN ELISA Kit; G3A ELISA Kit; HSPC336 ELISA Kit; MGC22400 ELISA Kit; NG20 ELISA Kit; NG20 like protein ELISA Kit; Protein G3a ELISA Kit
缩写：APOM
Uniprot No.：O95445
种属：Homo sapiens (Human)
样本类型：serum, plasma, cell culture supernates
检测范围：1.56 ng/mL-100 ng/mL
灵敏度：0.39 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human APOM in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1000 | Average % | 84 | ||||
| Range % | 80-96 | |||||
| 1:2000 | Average % | 95 | ||||
| Range % | 92-102 | |||||
| 1:4000 | Average % | 98 | ||||
| Range % | 92-110 | |||||
| 1:8000 | Average % | 95 | ||||
| Range % | 86-100 | |||||
回收率：
| The recovery of human APOM spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 92 | 87-98 | ||||
| EDTA plasma (n=4) | 96 | 92-102 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected 1002.5662.5472.5572.464 502.0111.9952.0031.910 251.4091.3941.4021.309 12.50.8670.8960.8820.789 6.250.5240.5020.5130.420 3.120.3450.3640.3550.262 1.560.2130.2170.2150.122 00.0940.0910.093 | ||||||
本试剂盒所含材料：
A micro ELISA plate --- The 96-well plate has been pre-coated with an anti-human ApoM antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
One vial Biotin-labeled ApoM antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
One vial Biotin-antibodyDiluent (15 ml/bottle) ---Dilute the Biotin-antibody.
One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.
本试剂盒不含材料：
A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
An incubator can provide stable incubation conditions up to 37°C±5°C.
Centrifuge
Vortex
Squirt bottle, manifold dispenser, or automated microplate washer
Absorbent paper for blotting the microtiter plate
货期：3-5 working days
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