首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人载脂蛋白L1(APOL1)酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆样本中的APOL1含量。APOL1是一种重要靶点。它与肾脏疾病等密切相关，APOL1基因变异增加肾脏疾病发生风险。研究机制方面，主要聚焦于其蛋白结构、功能，探索它在细胞内信号传导等过程作用，以及变异后如何影响肾脏细胞功能，为相关疾病治疗提供方向。试剂盒检测范围为7.81 ng/mL-500 ng/mL，该产品适用于基础医学研究领域，例如探究脂代谢异常相关疾病的分子机制、评估细胞模型中APOL1的表达调控，或分析组织样本中该蛋白的分布特征。为研究APOL1在代谢性疾病、感染免疫反应中的生物学功能提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：APO L ELISA Kit; Apo-L ELISA Kit; ApoL ELISA Kit; APOL I ELISA Kit; ApoL-I ELISA Kit; APOL1 ELISA Kit; APOL1_HUMAN ELISA Kit; APOLI ELISA Kit; Apolipoprotein L ELISA Kit; Apolipoprotein L I ELISA Kit; Apolipoprotein L-I ELISA Kit; Apolipoprotein L1 ELISA Kit; FSGS4 ELISA Kit
缩写：APOL1
Uniprot No.：O14791
种属：Homo sapiens (Human)
样本类型：serum, plasma, cell culture supernates, tissue homogenates
检测范围：7.81 ng/mL-500 ng/mL
灵敏度：1.95 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Metabolism
测定原理：quantitative
测定方法：Competitive
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human APOL1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:20 | Average % | 98 | |||||
| Range % | 92-106 | ||||||
| 1:40 | Average % | 93 | |||||
| Range % | 88-97 | ||||||
| 1:80 | Average % | 104 | |||||
| Range % | 102-107 | ||||||
| 1:160 | Average % | 88 | |||||
| Range % | 84-92 | ||||||
回收率：
| The recovery of human APOL1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 91 | 87-95 | |||||
| EDTA plasma (n=4) | 104 | 98-108 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| ng/mlOD1OD2Average5000.1450.1470.1462500.2410.2530.2471250.3830.3970.39062.50.6010.6130.60731.250.8570.8620.86015.621.1121.1551.1347.811.5971.6431.62001.9811.9991.990 | |||||||
货期：3-5 working days
×
