首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠伯胺氧化酶膜(AOC3)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的AOC3含量。AOC3又称血管粘附蛋白-1，是一种膜结合糖蛋白，在多种细胞中表达。与炎症、免疫反应等生理病理过程密切相关。研究机制方面，它能通过催化胺类氧化影响细胞黏附、迁移等，在炎症性疾病、心血管疾病等研究中有重要意义。试剂盒检测范围为7.8 ng/mL-500 ng/mL，适用于体外实验中对炎症相关疾病模型、药物干预效果或基因表达调控的科研分析，尤其适合探究AOC3在组织微环境中的动态变化及其分子调控网络，为炎症机制研究、靶向药物开发等基础研究提供可靠数据支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Aoc3; Membrane primary amine oxidase; Copper amine oxidase; Semicarbazide-sensitive amine oxidase; SSAO; VP97; Vascular adhesion protein 1; VAP-1
缩写：AOC3
Uniprot No.：O08590
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, tissue homogenates
检测范围：7.8 ng/mL-500 ng/mL
灵敏度：1.95 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Others
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat AOC3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 85 | ||||
| Range % | 84-94 | |||||
| 1:2 | Average % | 96 | ||||
| Range % | 91-102 | |||||
| 1:4 | Average % | 84 | ||||
| Range % | 81-88 | |||||
| 1:8 | Average % | 93 | ||||
| Range % | 88-99 | |||||
回收率：
| The recovery of rat AOC3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 92 | 87-97 | ||||
| EDTA plasma (n=4) | 94 | 91-97 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected5002.0752.0862.0811.9312501.5401.6691.6051.4551251.1111.1561.1340.98462.50.7580.7500.7540.60431.20.4370.4650.4510.30115.60.2610.2770.2690.1197.80.2110.2230.2170.06700.1490.1500.150 | ||||||
货期：3-5 working days
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