首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠伯胺氧化酶膜(AOC3)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的AOC3含量。AOC3作为靶点，是含氨氧化酶家族成员，主要分布在白细胞等细胞表面。其作用机制与细胞黏附、迁移等过程相关。目前研究聚焦于其在免疫调节、炎症反应等病理生理过程中的角色，有望为相关疾病的治疗提供新方向。试剂盒检测范围为1.25 ng/mL-80 ng/mL，本试剂盒适用于科研场景下的小鼠模型研究，如通过检测血清或血浆样本评估循环系统中AOC3的动态变化，或通过组织匀浆分析特定器官（如肝脏、脂肪组织）中该蛋白的表达特征，为炎症机制、药物干预效果或免疫调节研究提供定量分析工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Aoc3; Vap1; Membrane primary amine oxidase; Copper amine oxidase; Semicarbazide-sensitive amine oxidase; SSAO; Vascular adhesion protein 1; VAP-1
缩写：AOC3
Uniprot No.：O70423
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates
检测范围：1.25 ng/mL-80 ng/mL
灵敏度：0.31 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Others
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse AOC3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 83 | ||||
| Range % | 80-86 | |||||
| 1:2 | Average % | 90 | ||||
| Range % | 85-96 | |||||
| 1:4 | Average % | 96 | ||||
| Range % | 93-99 | |||||
| 1:8 | Average % | 104 | ||||
| Range % | 100-109 | |||||
回收率：
| The recovery of mouse AOC3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 90-100 | ||||
| EDTA plasma (n=4) | 97 | 94-100 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected802.5072.5692.5382.351401.6621.6001.6311.444200.9990.9680.9840.797100.5870.5720.5800.39350.4440.4580.4510.2642.50.3540.3620.3580.1711.250.2790.2980.2890.10200.1880.1860.187 | ||||||
货期：3-5 working days
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