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产品描述:人血管生成素相关蛋白7(ANGPTL7)酶联免疫试剂盒为双抗夹心法ELISA试剂盒,定量检测血清、血浆、组织匀浆样本中的ANGPTL7含量。ANGPTL7是一种血管生成素样蛋白,参与血管生成、炎症反应、内皮细胞损伤和糖脂代谢。研究发现,ANGPTL7在阻塞性睡眠呼吸暂停低通气综合征(OSAHS)患者中表达升高,可能与OSAHS的发生发展相关,并通过调节炎症反应、内皮损伤和脂代谢等机制影响疾病进程。试剂盒检测范围为0.312 ng/mL-20 ng/mL,该产品适用于基础医学研究中ANGPTL7在血管发育、代谢调控及病理状态下的动态变化分析,也可用于药物筛选模型中靶向干预效果的评估,为眼科疾病机制探索、代谢相关研究及生物标志物开发提供高效检测方案。本品仅用于科研,不用于临床诊断,产品具体参数及操作步骤详见产品说明书。
别名:Angiopoietin like 7 ELISA Kit; Angiopoietin like factor (CDT6) ELISA Kit; Angiopoietin like factor ELISA Kit; Angiopoietin related protein 7 ELISA Kit; Angiopoietin-like factor ELISA Kit; Angiopoietin-like protein 7 ELISA Kit; Angiopoietin-related protein 7 ELISA Kit; ANGL7_HUMAN ELISA Kit; ANGPTL7 ELISA Kit; AngX ELISA Kit; CDT6 ELISA Kit; Cornea derived transcript 6 protein ELISA Kit; Cornea-derived transcript 6 protein ELISA Kit; dJ647M16.1 ELISA Kit; OTTHUMP00000001986 ELISA Kit; RP4-647M16.2 ELISA Kit
缩写:ANGPTL7
Uniprot No.:O43827
种属:Homo sapiens (Human)
样本类型:serum, plasma, tissue homogenates
检测范围:0.312 ng/mL-20 ng/mL
灵敏度:0.078 ng/mL
反应时间:1-5h
样本体积:50-100ul
检测波长:450 nm
研究领域:Neuroscience
测定原理:quantitative
测定方法:Sandwich
精密度:
Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
Inter-assay Precision (Precision between assays): CV%<10% | |||||||
Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度:
To assess the linearity of the assay, samples were spiked with high concentrations of human ANGPTL7 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
Sample | Serum(n=4) | ||||||
1:1 | Average % | 99 | |||||
Range % | 92-104 | ||||||
1:2 | Average % | 87 | |||||
Range % | 82-93 | ||||||
1:4 | Average % | 92 | |||||
Range % | 87-98 | ||||||
1:8 | Average % | 101 | |||||
Range % | 98-105 |
回收率:
The recovery of human ANGPTL7 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
Sample Type | Average % Recovery | Range | |||||
Serum (n=5) | 103 | 92-106 | |||||
EDTA plasma (n=4) | 95 | 89-99 | |||||
标准曲线:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
ng/mlOD1OD2AverageCorrected201.9422.0421.9921.881101.2391.1491.1941.08350.7590.7190.7390.6282.50.4530.4640.4590.3481.250.3110.3210.3160.2050.6250.2120.2230.2180.1070.3120.1610.1650.1630.05200.1100.1110.111 |
货期:3-5 working days
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