| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/2000-1/5000 | Human,Mouse,Rat |
| Aliases | ARF1P2; ARL17P1; ARL17; ARL17A |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Fusion protein of human ARL17A/ARL17B |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于ARL17A/ARL17B抗体的3篇参考文献的简要整理(注：部分内容基于假设性文献，实际研究可能较少)：
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1. **文献名称**: *"Characterization of ARL17A/B in 17q12 Genomic Disorders"*
**作者**: Smith J. et al. (2020)
**摘要**: 本研究分析17q12染色体区域的结构变异与神经发育障碍的关联，发现ARL17A/B基因在该区域的重复或缺失可能影响细胞内囊泡运输。作者通过特异性抗体验证了ARL17A/B在患者细胞中的表达异常，提示其潜在病理机制。
2. **文献名称**: *"ARL17A/B Antibody Validation for Immunoblotting and Localization Studies"*
**作者**: Lee H. & Kim S. (2018)
**摘要**: 文章详细描述了针对ARL17A和ARL17B的多克隆抗体的开发与验证，证实其在Western blot和免疫荧光中的特异性，并发现两种蛋白主要定位于高尔基体和内体系统，支持其在膜运输中的功能。
3. **文献名称**: *"Functional Redundancy and Divergence in the ARL Protein Family"*
**作者**: Gonzalez R. et al. (2016)
**摘要**: 本研究通过比较ARL家族成员(包括ARL17A/B)的进化保守性和功能差异，发现ARL17A可能与线粒体动态调控相关，而ARL17B的表达受组织特异性调控，提示两者功能分化。抗体染色结果显示两者在脑组织中高表达。
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**备注**：
- ARL17A/B的研究相对有限，上述文献为示例性质，实际文献需通过PubMed或Google Scholar检索确认。
- 若需具体文献，可查询抗体供应商(如Abcam、Sigma)的产品说明书引用，或关注基因组结构变异相关研究。
**Background of ARL17A/ARL17B Antibodies**
ARL17A and ARL17B (ADP-ribosylation factor-like proteins 17A/B) are paralogous genes encoding small GTPases within the ARL family, which regulate intracellular trafficking and membrane dynamics. These genes, located in the 17q12 chromosomal region, are thought to have arisen through segmental duplication, resulting in high sequence homology. Due to their similarity, distinguishing between ARL17A and ARL17B proteins experimentally remains challenging, necessitating highly specific antibodies for accurate detection.
Antibodies targeting ARL17A/ARL17B are primarily used in research to explore their roles in cellular processes such as vesicle transport, cytoskeletal organization, and ciliogenesis. Dysregulation of these proteins has been implicated in neurodevelopmental disorders and cancers, particularly those linked to 17q12 copy number variations (e.g., renal cysts, diabetes syndrome). However, their precise molecular functions and disease associations remain understudied.
Most commercial ARL17A/ARL17B antibodies are polyclonal or monoclonal reagents validated for applications like Western blotting, immunofluorescence, or immunohistochemistry. Specificity validation often involves knockdown/knockout cell lines or peptide competition assays to confirm minimal cross-reactivity between paralogs or unrelated proteins. Challenges include potential cross-reactivity due to shared epitopes and limited functional characterization of isoform-specific roles.
Current research utilizes these antibodies to investigate tissue expression patterns, subcellular localization, and interactions with trafficking regulators like COPI/COPII complexes. Their development supports ongoing efforts to clarify ARL17A/B contributions to cellular homeostasis and disease mechanisms.
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