Rat angiotensin II (ANG-Ⅱ) ELISA kit

大包装询价

货号: IPD-E04494r

Price： ￥2500

48T 96T

数量：

大包装询价

产品描述：
大鼠血管紧张素Ⅱ(ANG-Ⅱ)酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆.样本中的ANG-Ⅱ含量。ANG-Ⅱ是重要的靶点。它是肾素-血管紧张素系统的关键效应肽。其作用机制主要是通过与受体结合，收缩血管、促进醛固酮分泌，影响血压调节、水盐平衡等。对它的研究有助于深入了解心血管疾病等病理生理过程，为相关治疗提供方向。试剂盒检测范围为15.6 pg/ml-1000 pg/ml.，该产品适用于基础科研场景，如心血管疾病动物模型研究、药物干预对血管活性物质的影响评估，或肾脏功能与体液平衡相关机制探索，为实验室提供稳定可靠的检测工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：
angiotensin II,ANG-Ⅱ,angiotensin 2,ANG-2
缩写：
ANG-Ⅱ
种属：
Rattus norvegicus (Rat)
样本类型：
serum, plasma.
检测范围：
15.6 pg/ml-1000 pg/ml.
灵敏度：
7.8 pg/ml.
反应时间：
1-5h
样本体积：
50-100ul
检测波长：
450 nm
研究领域：
Cardiovascular
测定原理：
quantitative
测定方法：
Competitive

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat ANG-Ⅱ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

	Sample	Serum(n=4)
1:1	Average %	84
	Range %	80-92
1:2	Average %	87
	Range %	81-95
1:4	Average %	90
	Range %	92-97
1:8	Average %	93
	Range %	86-98

回收率：

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.


pg/ml	OD1	OD2	Average
1000	0.158	0.160	0.159
500	0.185	0.189	0.187
250	0.237	0.232	0.235
125	0.364	0.340	0.352
62.5	0.595	0.568	0.581
31.25	0.958	0.902	0.930
15.6	1.355	1.298	1.326
0	2.287	2.126	2.206

Intra-assay Precision (Precision within an assay): CV%<12%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.