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产品描述：人血管紧张素Ⅰ(Ang-Ⅰ)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液样本中的Ang-Ⅰ含量。Ang-Ⅰ，即血管生成素-1（ANG-1），是一种分泌型糖蛋白，属于血管生成素家族。它作为酪氨酸激酶受体Tie-2的配体，主要作用于血管系统，控制血管的发育和稳定。Ang-Ⅰ在血管成熟过程中起重要作用，介导内皮细胞的迁移、粘附和存活，同时影响骨骼肌细胞并协调血管对组织损伤的反应。研究显示，Ang-Ⅰ在肿瘤生长中发挥关键作用，可促进肿瘤血管生成和肿瘤细胞增殖，抑制凋亡。试剂盒检测范围为15.6 pg/mL-1000 pg/mL，该产品可广泛应用于体外模型研究、药物干预实验及病理生理学分析等领域本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：angiotensin Ⅰ,Ang-Ⅰ,angiotensin 1,Ang-1
缩写：Ang-Ⅰ
种属：Homo sapiens (Human)
样本类型：serum, plasma, cell culture supernates
检测范围：15.6 pg/mL-1000 pg/mL
灵敏度：3.9 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cardiovascular
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human Ang-Ⅰ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 100 | ||||
| Range % | 97-105 | |||||
| 1:2 | Average % | 98 | ||||
| Range % | 94-103 | |||||
| 1:4 | Average % | 105 | ||||
| Range % | 100-110 | |||||
| 1:8 | Average % | 108 | ||||
| Range % | 102-114 | |||||
回收率：
| The recovery of human Ang-Ⅰ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 92 | 87-97 | ||||
| EDTA plasma (n=4) | 95 | 90-101 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected10002.6832.7522.7182.5675001.9051.9891.9471.7962501.0371.1221.0800.9291250.6310.6550.6430.49262.50.4310.4650.4480.29731.20.3070.3210.3140.16315.60.2300.2240.2270.07600.1470.1540.151 | ||||||
货期：3-5 working days
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