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Human RAC-alpha serine/threonine-protein kinase(AKT1) ELISA kit

  • 中文名： 人RACα丝氨酸/苏氨酸蛋白激酶(AKT1)ELISA kit
货号: IPD-EL001553HU
Price： ￥2500
数量：
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产品详情

  • 产品描述：
    人RACα丝氨酸/苏氨酸蛋白激酶(AKT1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、细胞裂解物样本中的AKT1含量。AKT1是一种丝氨酸/苏氨酸蛋白激酶，属于PI3K/AKT/mTOR信号通路的关键成员，参与调节细胞生长、存活、代谢等关键过程。其异常激活与多种癌症的发生发展密切相关，成为肿瘤治疗的重要靶点。试剂盒检测范围为0.312 ng/mL-20 ng/mL，适用于肿瘤机制研究、药物靶点筛选、信号通路调控分析等科研领域，尤其适合评估实验条件下AKT1蛋白在细胞模型或生物体液中的动态变化，为癌症生物学、分子药理学等研究提供可靠工具本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
  • 别名：
    AKT 1 ELISA Kit; AKT ELISA Kit; AKT1 ELISA Kit; AKT1_HUMAN ELISA Kit; C AKT ELISA Kit; cAKT ELISA Kit; MGC99656 ELISA Kit; PKB alpha ELISA Kit; PKB ELISA Kit; PKB-ALPHA ELISA Kit; PRKBA ELISA Kit; Protein Kinase B Alpha ELISA Kit; Protein kinase B ELISA Kit; Proto-oncogene c-Akt ELISA Kit; RAC Alpha ELISA Kit; RAC ELISA Kit; Rac protein kinase alpha ELISA Kit; RAC Serine/Threonine Protein Kinase ELISA Kit; RAC-alpha serine/threonine-protein kinase ELISA Kit; RAC-PK-alpha ELISA Kit; v akt murine thymoma viral oncogene homolog 1 ELISA Kit; vAKT Murine Thymoma Viral Oncogene Homolog 1 ELISA Kit
  • 缩写：
    AKT1
  • Uniprot No.：
    P31749
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, cell lysates
  • 检测范围：
    0.312 ng/mL-20 ng/mL
  • 灵敏度：
    0.078 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Signal Transduction
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%


    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%


    Three samples of known concentration were tested in twenty assays to assess.








  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human AKT1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

    		SampleSerum(n=4)
    1:1Average %96
    Range %93-99
    1:2Average %87
    Range %82-90
    1:4Average %101
    Range %98-104
    1:8Average %90
    Range %87-93
  • 回收率：
    The recovery of human AKT1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5)8582-89
    EDTA plasma (n=4)9390-96














    标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.



























































































    ng/mlOD1OD2AverageCorrected
    202.1492.1692.1592.015
    101.4441.4691.4571.313
    50.9180.9380.9280.784
    2.50.5770.5980.5880.444
    1.250.3880.3990.3940.250
    0.6250.3010.3210.3110.167
    0.3120.2530.2680.2610.117
    00.1430.1450.144









  • 货期：
    3-5 working days

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