首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人醛糖还原酶(AR)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、尿液、组织匀浆样本中的AKR1B1含量。AKR1B1 即醛酮还原酶家族 1 成员 B1，它在细胞内醛类等物质代谢中发挥重要作用，与糖尿病并发症、癌症等疾病密切相关。研究机制主要聚焦在其催化反应机制，以及其在疾病发生发展中信号通路相关作用，以探寻潜在治疗靶点。试剂盒检测范围为0.312 ng/mL-20 ng/mL，适用于基础科研领域中对糖尿病及其并发症机制的研究，亦可用于评估药物干预对 AR 活性的调控作用，或作为代谢性疾病模型中生物标志物的检测工具。可为细胞实验、动物模型及临床前研究提供可靠的定量数据支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：ADR ELISA Kit; AKR1B 1 ELISA Kit; Akr1b1 ELISA Kit; Aldehyde reductase 1 ELISA Kit; Aldehyde reductase ELISA Kit; Aldo keto reductase family 1; member B1 ELISA Kit; Aldo-keto reductase family 1 member B1 ELISA Kit; aldo-keto reductase family 1; member B1 (aldose reductase) ELISA Kit; Aldose reductase ELISA Kit; aldr 1 ELISA Kit; ALDR_HUMAN ELISA Kit; aldr1 ELISA Kit; ALR2 ELISA Kit; AR ELISA Kit; Lii5 2 CTCL tumor antigen ELISA Kit; Low Km aldose reductase ELISA Kit; MGC1804 ELISA Kit
缩写：AKR1B1
Uniprot No.：P15121
种属：Homo sapiens (Human)
样本类型：serum, plasma, urine, tissue homogenates
检测范围：0.312 ng/mL-20 ng/mL
灵敏度：0.078 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Metabolism
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human AR in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 86 | ||||
| Range % | 82-90 | |||||
| 1:2 | Average % | 94 | ||||
| Range % | 90-98 | |||||
| 1:4 | Average % | 87 | ||||
| Range % | 83-90 | |||||
| 1:8 | Average % | 100 | ||||
| Range % | 95-105 | |||||
回收率：
| The recovery of human AR spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 98 | 94-102 | ||||
| EDTA plasma (n=4) | 92 | 87-96 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected202.3922.3752.3842.222101.8591.8041.8321.67051.4111.4351.4231.2612.50.9660.9680.9670.8051.250.5400.5680.5540.3920.6250.3630.3680.3660.2040.3120.2350.2410.2380.07600.1640.1600.162 | ||||||
货期：3-5 working days
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