首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人凋亡诱导因子(AIF)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的AIFM1含量。AIFM1是一种与细胞凋亡相关的蛋白，其突变可能导致听神经病谱系障碍（ANSD）。研究显示，AIFM1突变影响凋亡诱导因子（AIF）的二聚化，导致细胞凋亡增加，进而引发ANSD。试剂盒检测范围为23.5 pg/mL-1500 pg/mL，该产品适用于基础科研中细胞凋亡机制探索、疾病模型构建、药物干预效果评估等场景，例如通过检测细胞凋亡过程中AIF的释放动态，或分析不同处理条件下组织样本的凋亡相关信号通路活性。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：AIFM1 ELISA Kit; AIFM1_HUMAN ELISA Kit; Apoptosis inducing factor 1, mitochondrial ELISA Kit; Apoptosis inducing factor ELISA Kit; Apoptosis inducing factor, mitochondrion associated, 1 ELISA Kit; Apoptosis-inducing factor 1 ELISA Kit; CMTX4 ELISA Kit; COWCK ELISA Kit; COXPD6 ELISA Kit; Harlequin ELISA Kit; Hq ELISA Kit; mAIF ELISA Kit; MGC111425 ELISA Kit; MGC5706 ELISA Kit; mitochondrial ELISA Kit; Neuropathy, axonal motor-sensory, with deafness and mental retardation ELISA Kit; neuropathy, axonal, motor-sensory with deafness and mental retardation (Cowchock syndrome) ELISA Kit; PDCD 8 ELISA Kit; PDCD8 ELISA Kit; Programmed cell death 8 (apoptosis inducing factor) ELISA Kit; Programmed cell death 8 ELISA Kit; Programmed cell death 8 isoform 1 ELISA Kit; Programmed cell death 8 isoform 2 ELISA Kit; Programmed cell death 8 isoform 3 ELISA Kit; Programmed cell death protein 8 ELISA Kit; Programmed cell death protein 8 mitochondrial ELISA Kit; Programmed cell death protein 8 mitochondrial precursor ELISA Kit; Programmed cell death protein 8 mitochondrial precursor ELISA Kit; Striatal apoptosis inducing factor ELISA Kit
缩写：AIFM1
Uniprot No.：O95831
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：23.5 pg/mL-1500 pg/mL
灵敏度：5.8 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cell Biology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human AIF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 92 | ||||
| Range % | 87-95 | |||||
| 1:2 | Average % | 89 | ||||
| Range % | 85-94 | |||||
| 1:4 | Average % | 88 | ||||
| Range % | 82-95 | |||||
| 1:8 | Average % | 98 | ||||
| Range % | 92-103 | |||||
回收率：
| The recovery of human AIF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 94 | 91-98 | ||||
| EDTA plasma (n=4) | 87 | 80-93 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected15002.3612.5452.4532.2957501.8061.7071.7571.5993751.0721.1541.1130.955187.50.6820.6730.6780.520940.4550.4430.4490.291470.2970.3050.3010.14323.50.2230.2290.2260.06800.1570.1580.158 | ||||||
货期：3-5 working days
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