| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/10-1/50 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/1000-1/2000 | Human,Mouse,Rat |
| Aliases | HSPC321; SWAP-70 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Fusion protein of human SWAP70 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于SWAP70抗体的3篇参考文献及其摘要内容：
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1. **文献名称**：*SWAP70 is essential for osmotic stress-induced actin cytoskeleton reorganization via its membrane translocation and PIP2 binding activity*
**作者**：Shinohara, M., Terada, Y., Iwamatsu, A., et al.
**摘要**：本研究利用SWAP70特异性抗体，通过免疫印迹和免疫荧光技术，揭示了SWAP70在细胞渗透压应激响应中的作用。结果显示，SWAP70通过结合磷脂酰肌醇二磷酸(PIP2)并易位至细胞膜，调控应激诱导的肌动蛋白骨架重排，抗体在实验中用于检测SWAP70的亚细胞定位及表达水平变化。
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2. **文献名称**：*SWAP70 functions as a Rac/Rho guanine nucleotide-exchange factor in B-cell antigen receptor-induced membrane ruffling*
**作者**：Matsuda, A., Suzuki, Y., Honda, G., et al.
**摘要**：该研究通过SWAP70抗体进行免疫共沉淀和体外GEF活性分析，证明SWAP70作为B细胞抗原受体信号通路中的Rac/Rho鸟苷酸交换因子，介导细胞膜皱褶形成。抗体在实验中用于验证SWAP70与Rac1的相互作用及其在信号转导中的功能。
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3. **文献名称**：*Critical role of SWAP70 in macrophages for colitis-associated colorectal cancer*
**作者**：Ortiz-Sánchez, E., Schulze, C.J., Bender, C., et al.
**摘要**：本文利用SWAP70基因敲除小鼠及特异性抗体，揭示了SWAP70在巨噬细胞中通过调控促炎细胞因子分泌促进结肠炎相关癌变。抗体用于流式细胞术和免疫组化分析，证实SWAP70在肿瘤微环境中巨噬细胞的表达上调及其病理意义。
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这些文献均涉及SWAP70抗体的实验应用，涵盖功能机制、信号通路及疾病关联研究。如需具体文章，建议通过PubMed或Sci-Hub输入标题检索全文。
SWAP70 (Switching B-cell complex 70 kDa subunit) is a unique signaling protein involved in B-cell antigen receptor signaling, immune regulation, and cytoskeletal dynamics. Discovered in 2000. it belongs to the SAC (SWAP-ACD) family and contains a pleckstrin homology (PH) domain for membrane binding and a SWAP domain mediating protein interactions. It regulates PI3K-Akt signaling, membrane ruffling, and cell migration in immune cells (B cells, dendritic cells) and epithelial cells.
SWAP70 antibodies are critical tools for studying its role in immune responses, inflammation, and autoimmune diseases (e.g., rheumatoid arthritis, allergies). They enable detection of SWAP70 expression, localization (cytosolic-to-membrane translocation upon activation), and interactions via techniques like Western blot, immunofluorescence, and flow cytometry. Research using these antibodies has linked SWAP70 dysfunction to cancer metastasis, immunodeficiency, and abnormal T-cell/B-cell activation. Commercial SWAP70 antibodies are typically raised against specific epitopes (human/mouse) and validated for cross-reactivity. Their applications span mechanistic studies of immune signaling, validation of SWAP70 knockout models, and clinical research exploring SWAP70 as a biomarker or therapeutic target.
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