首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人阿尔茨海默病相关神经丝蛋白(AD7C-NTP)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的AD7C-NTP含量。试剂盒检测范围为93.75 pg/mL-6000 pg/mL，灵敏度为23.43 pg/mL。适用于科研领域中对阿尔茨海默病发病机制的基础研究、药物开发过程中生物标志物动态监测，或探索脑脊液外其他体液样本中AD7C-NTP的表达水平。支持研究者对多种生物样本进行批量分析，为神经退行性疾病模型构建、病理分子网络解析等实验提供可靠数据支持本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：AD7C-NTP
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：93.75 pg/mL-6000 pg/mL
灵敏度：23.43 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Others
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of Human AD7C-NTP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:20 | Average % | 97 | ||||
| Range % | 93-101 | |||||
| 1:40 | Average % | 93 | ||||
| Range % | 86-97 | |||||
| 1:80 | Average % | 93 | ||||
| Range % | 87-97 | |||||
| 1:160 | Average % | 102 | ||||
| Range % | 98-106 | |||||
回收率：
| The recovery of Human AD7C-NTP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 98 | 94-102 | ||||
| EDTA plasma (n=4) | 87 | 82-93 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected 60002.6152.7872.7012.614 30002.2142.1152.1652.078 15001.4411.3861.4141.327 7500.7840.8210.8030.716 3750.4660.4570.4620.375 187.50.2740.2860.2800.193 93.750.1720.1660.1690.082 00.0860.0870.087 | ||||||
货期：3-5 working days
×
