首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠尿微量白蛋白(MAU/ALB)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、尿液样本中的MAU/ALB含量。MAU/ALB即尿微量白蛋白与尿肌酐比值，是反映早期肾脏损伤的敏感指标。其相关研究主要围绕通过检测该比值来评估肾脏微血管病变。因肾脏受损早期可出现微量白蛋白漏出，监测MAU/ALB有助于早发现肾脏疾病，干预治疗以延缓病情进展。试剂盒检测范围为0.01 μg/mL-40 μg/mL，灵敏度为0.01 μg/mL。微量白蛋白作为评估肾脏功能的重要生物标志物，其异常升高常提示早期肾小球滤过功能损伤，广泛应用于糖尿病肾病、高血压性肾损伤等代谢性疾病动物模型的科研领域。为研究大鼠肾脏疾病进展、药物干预效果及毒理学评价提供高效工具，特别适用于实验动物模型的高通量筛选及纵向研究。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Alb 1 ELISA Kit; alb ELISA Kit; ALBU_RAT ELISA Kit; Albumin ELISA Kit; Serum albumin ELISA Kit
缩写：MAU/ALB
Uniprot No.：P02770
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, urine
检测范围：0.01 μg/mL-40 μg/mL
灵敏度：0.01 μg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Metabolism
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat MAU in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1000 | Average % | 93 | ||||
| Range % | 85-107 | |||||
| 1:2000 | Average % | 95 | ||||
| Range % | 87-104 | |||||
| 1:4000 | Average % | 89 | ||||
| Range % | 84-100 | |||||
| 1:8000 | Average % | 90 | ||||
| Range % | 81-102 | |||||
回收率：
| The recovery of rat MAU spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 92 | 88-97 | ||||
| EDTA plasma (n=4) | 97 | 90-105 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| μg/mlOD1OD2AverageCorrected 402.3452.4122.3792.279 102.1762.2102.1932.093 2.51.6821.6571.6701.570 0.6251.2371.2531.2451.145 0.1560.7830.8020.7930.693 0.0390.4290.4360.4330.333 0.010.2720.2810.2770.177 00.1010.0980.100 | ||||||
货期：3-5 working days
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