首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人酪氨酸蛋白激酶受体UFO(AXL)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的AXL含量。AXL是一种受体酪氨酸激酶，异常表达与癌症患者的临床病理特征和预后不良有关。研究显示，AXL在肿瘤中参与增殖、迁移、侵袭等过程，与肿瘤不良预后及耐药密切相关。其研究机制涉及转录因子ETS1的调控，以及AXL抑制剂在癌症治疗中的潜力。试剂盒检测范围为31.25 pg/mL-2000 pg/mL，灵敏度为7.81 pg/mL。适用于科研领域中对AXL蛋白表达水平的精准评估，如癌症发生机制研究、纤维化疾病模型构建或炎症相关信号通路探索，其兼容的液体样本（血清/血浆）及组织匀浆样本可满足不同实验场景需求，为体外研究AXL蛋白功能及其调控机制提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：AXL
Uniprot No.：P30530
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：31.25 pg/mL-2000 pg/mL
灵敏度：7.81 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cancer
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human AXL in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 93 | ||||
| Range % | 84-95 | |||||
| 1:2 | Average % | 92 | ||||
| Range % | 85-99 | |||||
| 1:4 | Average % | 90 | ||||
| Range % | 86-95 | |||||
| 1:8 | Average % | 95 | ||||
| Range % | 85-100 | |||||
回收率：
| The recovery of human AXL spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 94 | 91-99 | ||||
| EDTA plasma (n=4) | 95 | 87-99 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected 20002.2322.2572.2452.075 10001.4621.5201.4911.321 5000.8590.8710.8650.695 2500.5270.5540.5410.371 1250.3250.3350.3300.160 62.50.2770.2590.2680.098 31.250.2210.2330.2270.057 00.1650.1750.170 | ||||||
货期：3-5 working days
×
