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产品描述：人NGAL/MMP-9复合物酶联免疫试剂盒检测血清、血浆、唾液、组织培养上清液、组织匀浆样本中的NGAL/MMP-9 complex含量。NGAL/MMP-9 complex 作为靶点备受关注。它在多种疾病如肿瘤、炎症等病理过程中发挥重要作用。研究机制上，二者形成复合物后可影响细胞生长、迁移、侵袭等行为，调节组织的微环境，通过干扰其相互作用有望为相关疾病治疗提供新思路。试剂盒检测范围为0.313 ng/mL-20 ng/mL，灵敏度为0.029 ng/mL。适用于基础医学研究中疾病机制探索、生物标志物分析及药物干预效果评估。为炎症相关疾病模型构建、肿瘤微环境研究及肾脏功能损伤实验提供高效工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：NGAL/MMP-9 complex
种属：Homo sapiens (Human)
样本类型：serum, plasma, saliva, cell culture supernates, tissue homogenates
检测范围：0.313 ng/mL-20 ng/mL
灵敏度：0.029 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Metabolism
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human NGAL/MMP-9 complex in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||
| Sample | Serum(n=4) | ||
| 1:10 | Average % | 91 | |
| Range % | 87-98 | ||
| 1:20 | Average % | 95 | |
| Range % | 90-102 | ||
| 1:40 | Average % | 93 | |
| Range % | 87-99 | ||
| 1:80 | Average % | 94 | |
| Range % | 86-105 |
回收率：
| The recovery of human NGAL/MMP-9 complex spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 99 | 94-103 | ||||
| EDTA plasma (n=4) | 94 | 87-100 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected 201.9552.0041.9801.857 101.6371.6291.6331.510 51.2041.2171.2111.088 2.50.8940.9070.9010.778 1.250.5730.5820.5780.455 0.6250.4160.4240.4200.297 0.3130.2810.2700.2760.153 00.1240.1210.123 | ||||||
货期：3-5 working days
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