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Mouse periostin/osteoblast specific factor 2 (POSTN) ELISA kit

  • 中文名： 小鼠骨膜蛋白/成骨细胞特异性因子2(POSTN) ELISA kit
货号: IPD-EL018381MO
Price： ￥2500
数量：
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产品详情

  • 产品描述：
    小鼠骨膜蛋白/成骨细胞特异性因子2(POSTN)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的POSTN含量。POSTN即骨膜蛋白，是一种细胞外基质蛋白。其在多种生理病理过程发挥作用，如胚胎发育、组织修复等。研究机制上，它可与多种细胞表面受体相互作用，激活下游信号通路，影响细胞增殖、迁移、黏附等，且在肿瘤等疾病中异常表达。试剂盒检测范围为1.56 ng/mL-100 ng/mL，灵敏度为0.039 ng/mL。适用于骨损伤修复、纤维化疾病模型（如肺纤维化、心肌纤维化）、肿瘤微环境研究等领域的机制探索，也可用于骨骼发育相关基因敲除或转基因动物模型中POSTN表达水平的动态监测。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
  • 别名：
    Postn ELISA Kit; Osf2 ELISA Kit; Periostin ELISA Kit; PN ELISA Kit; Osteoblast-specific factor 2 ELISA Kit; OSF-2 ELISA Kit
  • 缩写：
    POSTN
  • Uniprot No.：
    Q62009
  • 种属：
    Mus musculus (Mouse)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    1.56 ng/mL-100 ng/mL
  • 灵敏度：
    0.039 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Cardiovascular
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：

    Intra-assay Precision (Precision within an assay): CV%<8%




    Three samples of known concentration were tested twenty times on one plate to assess.


    Inter-assay Precision (Precision between assays): CV%<10%




    Three samples of known concentration were tested in twenty assays to assess.



    　Intra-Assay PrecisionInter-Assay PrecisionSample123123n202020202020Mean(ng/ml)12.56312.65212.75912.70612.54612.457SD0.0370.0350.0430.0580.0540.060CV(%)3.9783.7434.5706.1835.8136.494







  • 线性度：

    To assess the linearity of the assay, samples were spiked with high concentrations of mouse OSF-2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.


    Sample

    Serum(n=4)



    1:20

    Average %

    103



    Range %

    94-107



    1:40

    Average %

    96



    Range %

    85-100



    1:80

    Average %

    91



    Range %

    85-95



    1:160

    Average %

    91



    Range %

    82-94










  • 回收率：

    The recovery of mouse OSF-2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range%



    Serum (n=5)

    92

    84-96



    EDTA plasma (n=4)

    90

    83-95










  • 标准曲线：

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.








  • ng/ml

    OD1

    OD2

    Average

    Corrected



    100

    2.511

    2.435

    2.473

    2.299



    50

    2.101

    2.075

    2.088

    1.914



    25

    1.452

    1.499

    1.476

    1.302



    12.5

    0.925

    0.938

    0.932

    0.758



    6.25

    0.549

    0.573

    0.561

    0.387



    3.13

    0.402

    0.414

    0.408

    0.234



    1.56

    0.284

    0.293

    0.289

    0.115



    0

    0.172

    0.176

    0.174












  • 货期：
    3-5 working days

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