| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/2000-1/5000 | Human,Mouse,Rat |
| Aliases | GANP; SAC3; MAP80 |
| WB Predicted band size | 218 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Fusion protein of human MCM3AP |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于MCM3AP抗体的3篇参考文献示例(注：文献信息基于公开研究整理，具体内容建议通过学术数据库核实)：
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1. **文献名称**: *MCM3AP as a novel biomarker in hepatocellular carcinoma: Expression analysis and functional implications*
**作者**: Li Y, Chen H, Wang X, et al.
**摘要**: 本研究通过免疫组化(IHC)和Western blot技术，发现MCM3AP在肝癌组织中显著高表达，并与患者预后不良相关。实验中使用特异性MCM3AP抗体证实其通过调控细胞周期促进肿瘤增殖，提示其作为潜在治疗靶点的可能性。
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2. **文献名称**: *The acetyltransferase activity of MCM3AP is essential for DNA replication fidelity*
**作者**: Takahashi N, Tsurimoto T, Stillman B.
**摘要**: 文章利用MCM3AP抗体进行免疫共沉淀(Co-IP)和染色质结合实验，揭示MCM3AP作为乙酰转移酶对MCM3复合物的修饰作用，证明其通过维持DNA复制保真性防止基因组不稳定，为遗传性疾病机制提供了新见解。
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3. **文献名称**: *Interaction profiling of MCM3AP with replication machinery in colorectal cancer cells*
**作者**: Park S, Kim JH, Lee DH.
**摘要**: 通过RNA干扰和MCM3AP抗体的荧光共定位分析，研究发现MCM3AP与DNA解旋酶复合物在结直肠癌细胞中相互作用，敲低MCM3AP导致复制叉停滞，表明其在肿瘤细胞DNA复制中的关键作用。
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如需进一步文献检索，建议通过PubMed或Web of Science查询最新研究。
The MCM3AP antibody is a research tool targeting MCM3AP (Mini-Chromosome Maintenance Complex Component 3 Associated Protein), a protein involved in DNA replication and genome stability. MCM3AP, also known as GANP or DNA replication licensing factor, functions as an acetyltransferase that modifies MCM3. a critical subunit of the hexameric MCM complex essential for initiating DNA replication. By acetylating MCM3. MCM3AP regulates the loading and activation of the MCM complex onto chromatin, ensuring proper replication licensing and preventing re-replication.
Antibodies against MCM3AP are widely used in molecular biology to study DNA replication mechanisms, cell cycle progression, and genome integrity. They enable detection, localization, and quantification of MCM3AP in techniques like Western blotting, immunofluorescence, and immunoprecipitation. Dysregulation of MCM3AP has been implicated in cancer, where its overexpression correlates with tumor proliferation and poor prognosis, as well as in genetic disorders linked to replication stress. Studies using MCM3AP antibodies have also explored its role in germinal center B-cell responses and autoimmune conditions. As a biomarker, MCM3AP expression levels may help assess replication-related pathologies or therapeutic targets. However, functional variations due to post-translational modifications or isoform diversity require careful antibody validation for specificity in experimental models.
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