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Human carbonhydrate antigen 19-9 (CA19-9) ELISA kit

  • 中文名: 人糖类抗原19-9(CA19-9)ELISA kit
货号: IPD-E04773h
Price: ¥2500
数量:
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产品详情

  • 产品描述:
    人糖类抗原19-9(CA19-9)酶联免疫试剂盒为双抗夹心法ELISA试剂盒,定量检测血清、血浆样本中的CA19-9含量。CA19-9是一种肿瘤相关碳水化合物抗原,是临床常用的肿瘤标志物。其背景在于可作为胰腺癌、胆管癌等消化系统恶性肿瘤的辅助诊断指标。研究机制方面,主要是检测血清中CA19-9水平,当患有相关癌症时其含量可能异常升高。试剂盒检测范围为10 U/mL-150 U/mL,灵敏度为3.8 U/mL。该产品适用于体外培养模型、动物实验或临床前研究中 CA19 - 9 表达水平的动态监测,可用于肿瘤机制探索、药物疗效评估或生物标志物相关性分析等科研场景本品仅用于科研,不用于临床诊断,产品具体参数及操作步骤详见产品说明书。
  • 别名:
    CA199
  • 缩写:
    CA19-9
  • 种属:
    Homo sapiens (Human)
  • 样本类型:
    serum, plasma
  • 检测范围:
    10 U/mL-150 U/mL
  • 灵敏度:
    3.8 U/mL
  • 反应时间:
    1-5h
  • 样本体积:
    50-100ul
  • 检测波长:
    450 nm
  • 研究领域:
    Cancer
  • 测定原理:
    quantitative
  • 测定方法:
    Sandwich
  • 精密度:

    Intra-assay Precision (Precision within an assay): CV%<15%




    Three samples of known concentration were tested twenty times on one plate to assess.


    Inter-assay Precision (Precision between assays): CV%<20%




    Three samples of known concentration were tested in twenty assays to assess.



  • 线性度:

    To assess the linearity of the assay, samples were spiked with high concentrations of human CA19-9 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.


    Sample

    Serum(n=4)


    1:1

    Average %

    96


    Range %

    90-110


    1:2

    Average %

    89


    Range %

    85-99


    1:4

    Average %

    93


    Range %

    87-101


    1:8

    Average %

    95


    Range %

    89-98


  • 回收率:

    The recovery of human CA19-9 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range


    Serum (n=5)

    97

    93-100


    EDTA plasma (n=4)

    97

    90-101


  • 标准曲线:

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.







    U/ml

    OD1

    OD2

    Average



    150

    2.045

    2.011

    2.028



    100

    1.292

    1.300

    1.296



    40

    0.728

    0.735

    0.732



    10

    0.304

    0.306

    0.305




  • 货期:
    3-5 working days

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