| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/2000-1/5000 | Human,Mouse,Rat |
| Aliases | MYD118; GADD45BETA |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Fusion protein of human GADD45B |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于GADD45B抗体的3篇参考文献示例(注：文献信息为模拟示例，实际引用需核实)：
1. **文献名称**: "GADD45B regulates DNA damage-induced apoptosis via p38 MAPK signaling"
**作者**: Tamura, R.E., et al.
**摘要**: 本研究利用GADD45B特异性抗体，通过Western blot和免疫荧光技术，揭示了GADD45B在DNA损伤剂(如紫外线)作用下通过激活p38 MAPK通路调控细胞凋亡的机制。
2. **文献名称**: "Expression of GADD45B in neurodegenerative disorders: Insights from post-mortem brain analysis"
**作者**: Smith, M.A., et al.
**摘要**: 通过免疫组化(IHC)和qPCR，作者发现阿尔茨海默病患者脑组织中GADD45B蛋白表达显著上调，提示其可能参与神经退行性病变中的氧化应激反应。研究中使用了高特异性GADD45B抗体进行组织定位。
3. **文献名称**: "GADD45B as a prognostic marker in hepatocellular carcinoma: Immunohistochemical validation"
**作者**: Zhang, L., et al.
**摘要**: 该研究在肝癌患者组织样本中，采用GADD45B抗体进行免疫组化分析，发现低表达GADD45B与患者不良预后相关，表明其可能作为潜在的肿瘤抑制因子。
4. **文献名称**: "GADD45B mediates autophagy in response to chemotherapeutic stress"
**作者**: Jones, S.J., et al.
**摘要**: 通过siRNA敲低和GADD45B抗体检测，研究证实GADD45B在化疗药物诱导的细胞自噬中起关键作用，其表达水平与自噬标志物LC3-II呈正相关。
建议通过PubMed或Web of Science以“GADD45B antibody”或“GADD45B expression”为关键词检索最新文献获取准确信息。
The GADD45B (Growth Arrest and DNA Damage-Inducible Beta) antibody is a crucial tool for studying the GADD45B protein, a member of the GADD45 family involved in cellular stress responses. GADD45B is induced by various stressors, including DNA damage, oxidative stress, and cytokine signaling, and plays roles in cell cycle arrest, apoptosis, DNA repair, and inflammation. It interacts with key signaling pathways such as p38 MAPK and NF-κB, influencing processes like tumor suppression, immune regulation, and tissue homeostasis.
Antibodies targeting GADD45B enable researchers to detect and quantify its expression in cells and tissues using techniques like Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF). These antibodies are essential for exploring GADD45B's involvement in diseases such as cancer, neurodegenerative disorders, and autoimmune conditions. For instance, altered GADD45B expression has been linked to tumor progression, neuronal survival, and inflammatory responses.
Validated GADD45B antibodies typically undergo specificity testing (e.g., knockout controls) to ensure accurate detection. Researchers prioritize antibodies with high sensitivity and cross-reactivity across species (e.g., human, mouse, rat) for translational studies. Commercial antibodies often include validation data in datasheets, supporting their reliability in experimental models. Understanding GADD45B's regulation and function via these antibodies provides insights into stress adaptation mechanisms and potential therapeutic targets.
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