| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | NDUFV3 |
| Uniprot No | P56181 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 35-108aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMGSSAESGKSEKGQPQNSKKQSPPKKPAPV PAEPFDNTTYKNLQHHDYSTYTFLDLNLELSKFRMPQPSSGRESPRH |
| 预测分子量 | 11 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于NDUFV3重组蛋白的参考文献示例(注：部分文献信息为假设性概括，实际文献可能需要进一步检索验证)：
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1. **文献名称**: "Structural Insights into the NDUFV3 Subunit of Mitochondrial Complex I through Recombinant Protein Expression"
**作者**: Smith J, et al.
**摘要**: 本研究通过大肠杆菌表达系统成功重组了人源NDUFV3蛋白，并利用X射线晶体学解析其三维结构。结果表明，NDUFV3在复合物I的电子传递链中参与黄素单核苷酸(FMN)的结合，其重组蛋白的稳定性为研究复合物I功能障碍相关疾病提供了新工具。
2. **文献名称**: "Functional Characterization of NDUFV3 Mutations in Leigh Syndrome Using Recombinant Protein Models"
**作者**: Chen L, et al.
**摘要**: 通过构建NDUFV3重组蛋白的致病突变体(如p.Arg228Cys)，作者发现突变导致蛋白与复合物I其他亚基(如NDUFS1)的相互作用减弱，并显著降低NADH脱氢酶活性。该研究为NDUFV3突变相关线粒体疾病的分子机制提供了实验证据。
3. **文献名称**: "Optimized Expression and Purification of Recombinant NDUFV3 for Drug Screening Applications"
**作者**: Gupta R, et al.
**摘要**: 本文开发了一种基于昆虫细胞-杆状病毒系统的NDUFV3重组蛋白高效表达与纯化方案。纯化后的蛋白可用于高通量药物筛选，以寻找调控复合物I功能的潜在化合物，尤其针对神经退行性疾病。
4. **文献名称**: "NDUFV3 Recombinant Protein Interaction Network Analysis by Surface Plasmon Resonance"
**作者**: Müller T, et al.
**摘要**: 利用表面等离子共振技术(SPR)，作者系统分析了重组NDUFV3蛋白与复合物I核心亚基(如NDUFS2、NDUFS3)的动态结合特性，揭示了其在复合物组装中的关键作用，并为相关基因编辑研究提供了定量数据支持。
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**注意**：上述文献为示例性内容，实际研究中请通过PubMed、Web of Science等数据库检索真实文献。若需具体文献指引，建议结合关键词“NDUFV3 recombinant”“complex I assembly”进一步筛选。
NDUFV3 (NADH:ubiquinone oxidoreductase core subunit V3) is a nuclear-encoded component of mitochondrial Complex I, the largest enzyme in the electron transport chain responsible for oxidative phosphorylation. As part of the flavoprotein subcomplex, NDUFV3 plays a critical role in electron transfer from NADH to ubiquinone, contributing to proton gradient formation and ATP synthesis. Mutations in NDUFV3 have been linked to mitochondrial disorders, including Leigh syndrome and cardiomyopathy, highlighting its functional importance in cellular energy metabolism.
Recombinant NDUFV3 protein is engineered to study its structural, functional, and pathological characteristics. Produced through heterologous expression systems (e.g., E. coli or mammalian cells), the recombinant form enables researchers to investigate protein-protein interactions within Complex I, map mutation-induced conformational changes, and explore therapeutic interventions. Its applications extend to disease modeling, drug screening for mitochondrial disorders, and antibody development for diagnostic assays. Recent cryo-EM studies using recombinant subunits have advanced understanding of Complex I assembly and dysfunction mechanisms. However, challenges persist in maintaining proper folding and post-translational modifications during recombinant production, necessitating optimization of expression protocols. Current research focuses on leveraging recombinant NDUFV3 to develop targeted therapies and biomarkers for mitochondrial diseases.
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