首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠肿瘤坏死因子可溶性受体Ⅱ(TNFsR-Ⅱ)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的TNFRSF1B含量。TNFRSF1B，也称为TNFR2或CD120b，是肿瘤坏死因子受体超家族的成员，参与肿瘤坏死因子（TNF）信号传导，影响细胞生存和增殖。研究显示，TNFRSF1B在多种肿瘤类型的耗竭T细胞中高表达，可能成为肿瘤免疫治疗的潜在靶点。试剂盒检测范围为7.8125 pg/mL-500 pg/mL，灵敏度为1.6 pg/mL。广泛应用于科研领域，包括但不限于炎症性疾病模型研究、免疫调节机制探索、肿瘤微环境分析以及药物开发过程中生物标志物动态监测，为体外实验中TNFsR-Ⅱ表达水平的定量评估提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Tnfrsf1b; Tnfr-2; Tnfr2; Tumor necrosis factor receptor superfamily member 1B; Tumor necrosis factor receptor 2; TNF-R2; Tumor necrosis factor receptor type II; TNF-RII; TNFR-II; p75; p80 TNF-alpha receptor; CD antigen CD120b
缩写：TNFRSF1B
Uniprot No.：P25119
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates
检测范围：7.8125 pg/mL-500 pg/mL
灵敏度：1.6 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse TNFsR-Ⅱ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:20 | Average % | 91 | |||||
| Range % | 86-95 | ||||||
| 1:40 | Average % | 97 | |||||
| Range % | 92-102 | ||||||
| 1:80 | Average % | 90 | |||||
| Range % | 87-98 | ||||||
| 1:160 | Average % | 92 | |||||
| Range % | 85-96 | ||||||
回收率：
| The recovery of mouse TNFsR-Ⅱ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 93 | 89-97 | |||||
| EDTA plasma (n=4) | 98 | 95-101 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| pg/mlOD1OD2AverageCorrected 5002.8582.9112.8852.777 2502.3602.2502.3052.197 1251.4851.4551.4701.362 62.50.7850.8120.7990.691 31.250.4820.5210.5020.394 15.6250.2700.2630.2670.159 7.81250.1890.1870.1880.080 00.1040.1120.108 | |||||||
货期：3-5 working days
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