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Human soluble tumor necrosis factor receptor 2,sTNF-R2 ELISA Kit

  • 中文名: 人可溶性肿瘤坏死因子受体2(sTNF-R2)ELISA Kit
货号: IPD-E11266h
Price: ¥2500
数量:
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产品详情

  • 产品描述:人可溶性肿瘤坏死因子受体2(sTNF-R2)酶联免疫试剂盒为双抗夹心法ELISA试剂盒,定量检测血清、血浆、组织匀浆样本中的TNFRSF1B含量。TNFRSF1B,也称为TNFR2或CD120b,是肿瘤坏死因子受体超家族的成员,主要表达在肿瘤微环境中的Treg细胞和多种肿瘤细胞中。研究显示,TNFRSF1B在多种肿瘤类型的耗竭T细胞中高表达,其高表达会影响患者预后及生存周期。研究机制方面,TNFRSF1B基因高表达会抑制T细胞分泌IFN-γ,进而影响T细胞活性和耗竭功能。试剂盒检测范围为31.25 pg/mL-2000 pg/mL,灵敏度为7.8 pg/mL。适用于基础科研中炎症性疾病模型评估、免疫调控通路研究以及药物干预效果分析等场景。其兼容多种生物样本的特性,为探索不同组织或体液中的sTNF - R2表达差异提供了灵活方案,可用于体外实验条件下的信号通路解析或生物标志物筛选,助力科研人员深入探究TNF信号网络在病理生理中的作用机制。本品仅用于科研,不用于临床诊断,产品具体参数及操作步骤详见产品说明书。

  • 别名:CD120b ELISA Kit; p75 ELISA Kit; p75 TNF receptor ELISA Kit; p75TNFR ELISA Kit; p80 TNF alpha receptor ELISA Kit; p80 TNF-alpha receptor ELISA Kit; Soluble TNFR1B variant 1 ELISA Kit; TBP-2 ELISA Kit; TBPII ELISA Kit; TNF R II ELISA Kit; TNF R2 ELISA Kit; TNF R75 ELISA Kit; TNF-R2 ELISA Kit; TNF-RII ELISA Kit; TNFBR ELISA Kit; TNFR-II ELISA Kit; TNFR1B ELISA Kit; TNFR2 ELISA Kit; TNFR80 ELISA Kit; TNFRII ELISA Kit; Tnfrsf1b ELISA Kit; TNR1B_HUMAN ELISA Kit; Tumor necrosis factor beta receptor ELISA Kit; Tumor necrosis factor binding protein 2 ELISA Kit; Tumor necrosis factor receptor 2 ELISA Kit; Tumor necrosis factor receptor superfamily member 1B ELISA Kit; Tumor necrosis factor receptor type II ELISA Kit; Tumor necrosis factor-binding protein 2 ELISA Kit

  • 缩写:TNFRSF1B

  • Uniprot No.:P20333

  • 种属:Homo sapiens (Human)

  • 样本类型:serum, plasma, tissue homogenates

  • 检测范围:31.25 pg/mL-2000 pg/mL

  • 灵敏度:7.8 pg/mL

  • 反应时间:1-5h

  • 样本体积:50-100ul

  • 检测波长:450 nm

  • 研究领域:Signal Transduction

  • 测定原理:quantitative

  • 测定方法:Sandwich

  • 精密度:

    Intra-assay Precision (Precision within an assay): CV%<8%



    Three samples of known concentration were tested twenty times on one plate to assess.

    Inter-assay Precision (Precision between assays): CV%<10%



    Three samples of known concentration were tested in twenty assays to assess.










  • 线性度:

    To assess the linearity of the assay, samples were spiked with high concentrations of human sTNF-R2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

    SampleSerum(n=4)

    1:1Average %101

    Range %95-105

    1:2Average %93

    Range %87-97

    1:4Average %97

    Range %85-101

    1:8Average %94

    Range %85-99

  • 回收率:

    The recovery of human sTNF-R2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample TypeAverage % RecoveryRange

    Serum (n=5)10094-104

    EDTA plasma (n=4)10192-104

















  • 标准曲线:

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

    pg/mlOD1OD2AverageCorrected  20002.1702.0992.1352.032  10001.7211.7881.7551.652  5001.2721.2431.2581.155  2500.7760.8070.7920.689  1250.4870.5010.4940.391  62.50.3060.3220.3140.211  31.250.2090.2130.2110.108  00.1020.1040.103   







  • 货期:3-5 working days

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