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产品描述:人可溶性肿瘤坏死因子受体2(sTNF-R2)酶联免疫试剂盒为双抗夹心法ELISA试剂盒,定量检测血清、血浆、组织匀浆样本中的TNFRSF1B含量。TNFRSF1B,也称为TNFR2或CD120b,是肿瘤坏死因子受体超家族的成员,主要表达在肿瘤微环境中的Treg细胞和多种肿瘤细胞中。研究显示,TNFRSF1B在多种肿瘤类型的耗竭T细胞中高表达,其高表达会影响患者预后及生存周期。研究机制方面,TNFRSF1B基因高表达会抑制T细胞分泌IFN-γ,进而影响T细胞活性和耗竭功能。试剂盒检测范围为31.25 pg/mL-2000 pg/mL,灵敏度为7.8 pg/mL。适用于基础科研中炎症性疾病模型评估、免疫调控通路研究以及药物干预效果分析等场景。其兼容多种生物样本的特性,为探索不同组织或体液中的sTNF - R2表达差异提供了灵活方案,可用于体外实验条件下的信号通路解析或生物标志物筛选,助力科研人员深入探究TNF信号网络在病理生理中的作用机制。本品仅用于科研,不用于临床诊断,产品具体参数及操作步骤详见产品说明书。
别名:CD120b ELISA Kit; p75 ELISA Kit; p75 TNF receptor ELISA Kit; p75TNFR ELISA Kit; p80 TNF alpha receptor ELISA Kit; p80 TNF-alpha receptor ELISA Kit; Soluble TNFR1B variant 1 ELISA Kit; TBP-2 ELISA Kit; TBPII ELISA Kit; TNF R II ELISA Kit; TNF R2 ELISA Kit; TNF R75 ELISA Kit; TNF-R2 ELISA Kit; TNF-RII ELISA Kit; TNFBR ELISA Kit; TNFR-II ELISA Kit; TNFR1B ELISA Kit; TNFR2 ELISA Kit; TNFR80 ELISA Kit; TNFRII ELISA Kit; Tnfrsf1b ELISA Kit; TNR1B_HUMAN ELISA Kit; Tumor necrosis factor beta receptor ELISA Kit; Tumor necrosis factor binding protein 2 ELISA Kit; Tumor necrosis factor receptor 2 ELISA Kit; Tumor necrosis factor receptor superfamily member 1B ELISA Kit; Tumor necrosis factor receptor type II ELISA Kit; Tumor necrosis factor-binding protein 2 ELISA Kit
缩写:TNFRSF1B
Uniprot No.:P20333
种属:Homo sapiens (Human)
样本类型:serum, plasma, tissue homogenates
检测范围:31.25 pg/mL-2000 pg/mL
灵敏度:7.8 pg/mL
反应时间:1-5h
样本体积:50-100ul
检测波长:450 nm
研究领域:Signal Transduction
测定原理:quantitative
测定方法:Sandwich
精密度:
Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
Inter-assay Precision (Precision between assays): CV%<10% | |||||||
Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度:
To assess the linearity of the assay, samples were spiked with high concentrations of human sTNF-R2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
Sample | Serum(n=4) | ||||||
1:1 | Average % | 101 | |||||
Range % | 95-105 | ||||||
1:2 | Average % | 93 | |||||
Range % | 87-97 | ||||||
1:4 | Average % | 97 | |||||
Range % | 85-101 | ||||||
1:8 | Average % | 94 | |||||
Range % | 85-99 |
回收率:
The recovery of human sTNF-R2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
Sample Type | Average % Recovery | Range | |||||
Serum (n=5) | 100 | 94-104 | |||||
EDTA plasma (n=4) | 101 | 92-104 | |||||
标准曲线:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
pg/mlOD1OD2AverageCorrected 20002.1702.0992.1352.032 10001.7211.7881.7551.652 5001.2721.2431.2581.155 2500.7760.8070.7920.689 1250.4870.5010.4940.391 62.50.3060.3220.3140.211 31.250.2090.2130.2110.108 00.1020.1040.103 |
货期:3-5 working days
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