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产品描述：人肿瘤坏死因子可溶性受体Ⅰ(TNFsR-Ⅰ)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、尿液样本中的TNFRSF1A含量。TNFRSF1A是重要靶点。它是肿瘤坏死因子受体超家族成员，在炎症、免疫调节等生理病理过程起关键作用。研究机制围绕其与配体结合激活多条信号通路，调控细胞存活、凋亡等，为相关疾病治疗药物研发提供方向。试剂盒检测范围为78.125 pg/mL-5000 pg/mL，灵敏度为65 pg/mL。适用于体外实验中对炎症相关信号通路、药物干预效果评估或疾病分子机制的基础研究。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：CD120a ELISA Kit; FPF ELISA Kit; MGC19588 ELISA Kit; p55 ELISA Kit; p55-R ELISA Kit; p60 ELISA Kit; TBP1 ELISA Kit; TBPI ELISA Kit; TNF R ELISA Kit; TNF R55 ELISA Kit; TNF-R1 ELISA Kit; TNF-RI ELISA Kit; TNFAR ELISA Kit; TNFR-I ELISA Kit; TNFR1 ELISA Kit; TNFR55 ELISA Kit; TNFR60 ELISA Kit; TNFRI ELISA Kit; TNFRSF1a ELISA Kit; TNR1A_HUMAN ELISA Kit; Tumor necrosis factor receptor 1 ELISA Kit; Tumor necrosis factor receptor superfamily, member 1A ELISA Kit; Tumor necrosis factor receptor type 1 ELISA Kit; Tumor necrosis factor receptor type I ELISA Kit; Tumor necrosis factor-binding protein 1 ELISA Kit
缩写：TNFRSF1A
Uniprot No.：P19438
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, urine
检测范围：78.125 pg/mL-5000 pg/mL
灵敏度：65 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human TNFsR-Ⅰ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 98 | |||||
| Range % | 92-105 | ||||||
| 1:2 | Average % | 91 | |||||
| Range % | 86-98 | ||||||
| 1:4 | Average % | 91 | |||||
| Range % | 88-96 | ||||||
| 1:8 | Average % | 101 | |||||
| Range % | 96-106 | ||||||
回收率：
| The recovery of human TNFsR-Ⅰ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 90 | 87-93 | |||||
| EDTA plasma (n=4) | 101 | 103-104 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| pg/mlOD1OD2AverageCorrected 50002.5682.6322.6002.450 25002.2452.1212.1832.033 12501.6221.7411.6821.532 6250.9770.9550.9660.816 312.50.6600.6260.6430.493 156.250.3920.3660.3790.229 78.1250.2610.2540.2580.108 00.1450.1540.150 | |||||||
货期：3-5 working days
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