Human Tumor necrosis factor receptor superfamily member 14(TNFRSF14) ELISA kit

中文名： 人肿瘤坏死因子配体超家族成员14(TNFSF14)酶联免疫试剂盒

货号: IPD-EL023973HU

Price： ￥2500

48T 96T

数量：

大包装询价

产品详情

产品描述：
人肿瘤坏死因子配体超家族成员14(TNFSF14)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的TNFRSF14含量。TNFRSF14属肿瘤坏死因子受体超家族，在免疫调节等方面有重要作用。其研究机制主要聚焦免疫逃逸和肿瘤微环境调控，它可与配体结合激活信号通路，影响免疫细胞功能，是潜在的肿瘤免疫治疗靶点，相关研究为抗癌治疗带来新方向。试剂盒检测范围为15.6 pg/mL-1000 pg/mL，灵敏度为3.9 pg/mL。适用于细胞因子网络研究、肿瘤免疫微环境分析及炎症性疾病机制探索等科研领域，例如在动物模型或体外实验中评估TNFSF14表达水平与疾病进展的关联性，或作为免疫治疗相关基础研究的定量工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：
HVEML ELISA Kit; ATAR ELISA Kit; CD270 ELISA Kit; CD40 like protein precursor ELISA Kit; Herpes virus entry mediator A ELISA Kit; Herpesvirus entry mediator A ELISA Kit; Herpesvirus entry mediator ELISA Kit; Herpesvirus entry mediator ligand ELISA Kit; HveA ELISA Kit; HVEM ELISA Kit; HVEM L ELISA Kit; LIGHT ELISA Kit; LIGHTR ELISA Kit; TNFRSF14 ELISA Kit; TNFSF 14 ELISA Kit; TNR14_HUMAN ELISA Kit; TR2 ELISA Kit; Tumor necrosis factor receptor like gene2 ELISA Kit; Tumor necrosis factor receptor superfamily member 14 ELISA Kit; Tumor necrosis factor receptor superfamily member 14 precursor ELISA Kit; Tumor necrosis factor receptor-like 2 ELISA Kit
缩写：
TNFRSF14
Uniprot No.：
Q92956
种属：
Homo sapiens (Human)
样本类型：
serum, plasma, tissue homogenates
检测范围：
15.6 pg/mL-1000 pg/mL
灵敏度：
3.9 pg/mL
反应时间：
1-5h
样本体积：
50-100ul
检测波长：
450 nm
研究领域：
Immunology
测定原理：
quantitative
测定方法：
Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Intra-Assay PrecisionInter-Assay PrecisionSample123123n202020202020Mean(pg/ml)133.445127.689126.259121.987125.188124.119SD0.0290.0250.0230.0350.0390.034CV(%)5.2064.6214.2836.6677.3036.403

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human TNFRSF14 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

	Sample	Serum(n=4)
1:1	Average %	88
	Range %	85-99
1:2	Average %	95
	Range %	89-103
1:4	Average %	93
	Range %	89-101
1:8	Average %	90
	Range %	85-98

回收率：

The recovery of human TNFRSF14 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	89	83-95
EDTA plasma (n=4)	93	85-101

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.


pg/ml	OD1	OD2	Average	Corrected
1000	2.243	2.148	2.196	2.030
500	1.379	1.352	1.366	1.200
250	0.824	0.904	0.864	0.698
125	0.522	0.547	0.535	0.369
62.5	0.309	0.316	0.313	0.147
31.2	0.247	0.232	0.240	0.074
15.6	0.198	0.201	0.200	0.034
0	0.166	0.165	0.166