Human tissue inhibitors of metalloproteinase 3,TIMP-3 ELISA Kit

中文名： 人基质金属蛋白酶抑制因子3(TIMP-3)ELISA Kit

货号: IPD-E04734h

Price： ￥2500

48T 96T

数量：

大包装询价

产品详情

产品描述：
人基质金属蛋白酶抑制因子3(TIMP-3)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的TIMP3含量。TIMP3 即金属蛋白酶组织抑制因子 3，是内源性 MMP 抑制剂。它能与 MMPs 活性中心的锌离子结合，抑制 MMPs 活性。其在肿瘤、心血管疾病等多种疾病中表达异常，通过调节细胞外基质代谢、抑制血管生成等机制参与疾病发生发展。试剂盒检测范围为31.25 pg/ml-2000 pg/ml，灵敏度为7.8 pg/ml。为研究TIMP - 3在疾病模型中的表达调控、药物干预效果评估以及分子机制探索提供可靠工具，广泛应用于细胞生物学、病理学及药理学等科研领域，尤其适用于涉及组织损伤修复、肿瘤微环境或代谢性疾病的基础研究。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：
HSMRK222 ELISA Kit; K222 ELISA Kit; K222TA2 ELISA Kit; Metalloproteinase inhibitor 3 ELISA Kit; MIG 5 protein ELISA Kit; MIG5 protein ELISA Kit; Protein MIG 5 ELISA Kit; Protein MIG-5 ELISA Kit; SFD ELISA Kit; Sorsby fundus dystrophy pseudoinflammatory ELISA Kit; TIMP 3 ELISA Kit; TIMP metallopeptidase inhibitor 3 ELISA Kit; TIMP-3 ELISA Kit; TIMP3 ELISA Kit; TIMP3_HUMAN ELISA Kit; Tissue Inhibitor of Metalloproteinase 3 ELISA Kit; Tissue inhibitor of metalloproteinases 3 ELISA Kit; Tissue inhibitor of metalloproteinases3 ELISA Kit
缩写：
TIMP3
Uniprot No.：
P35625
种属：
Homo sapiens (Human)
样本类型：
serum, plasma, tissue homogenates
检测范围：
31.25 pg/ml-2000 pg/ml
灵敏度：
7.8 pg/ml
反应时间：
1-5h
样本体积：
50-100ul
检测波长：
450 nm
研究领域：
Cancer
测定原理：
quantitative
测定方法：
Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean(pg/ml)	246.660	246.505	242.560	248.749	245.363	251.011
SD	0.038	0.035	0.046	0.034	0.033	0.031
CV(%)	6.221	5.710	7.590	5.574	5.464	5.049

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human TIMP-3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

Sample

Serum(n=4)

1:4

Average %

Range %

87-98

1:8

Average %

Range %

85-94

1:16

Average %

Range %

88-93

1:32

Average %

Range %

89-98

回收率：

The recovery of human TIMP-3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range%
Serum (n=5)	91	87-94
EDTA plasma (n=4)	95	88-97

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.











pg/ml	OD1	OD2	Average	Corrected
2000	2.753	3.081	2.917	2.761
1000	1.636	1.796	1.716	1.560
500	1.030	1.088	1.059	0.903
250	0.541	0.565	0.553	0.397
125	0.390	0.365	0.378	0.222
62.5	0.261	0.247	0.254	0.098
31.25	0.202	0.215	0.209	0.053
0	0.154	0.157	0.156