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产品描述:大鼠基质金属蛋白酶抑制因子1(TIMP-1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒,定量检测血清、血浆、组织培养上清液、组织匀浆样本中的TIMP1含量。TIMP1 即组织金属蛋白酶抑制剂 1,它在细胞外基质重塑、肿瘤发展等过程中扮演重要角色。研究显示,其可抑制基质金属蛋白酶活性,影响肿瘤细胞的增殖、侵袭和转移。还参与调节细胞生长、凋亡等多种生理病理机制,是肿瘤和其他疾病研究的潜在靶点。试剂盒检测范围为0.156 ng/mL-10 ng/mL,灵敏度为0.224 ng/mL。广泛应用于心血管疾病模型、肝肺纤维化研究、肿瘤侵袭性分析等科研领域,尤其适用于动态监测细胞培养体系中TIMP - 1的分泌水平或评估组织样本中蛋白酶抑制系统的活性变化。本品仅用于科研,不用于临床诊断,产品具体参数及操作步骤详见产品说明书。
别名:Timp1 ELISA Kit; Timp-1 ELISA Kit; Metalloproteinase inhibitor 1 ELISA Kit; Tissue inhibitor of metalloproteinases 1 ELISA Kit; TIMP-1 ELISA Kit
缩写:TIMP1
Uniprot No.:P30120
种属:Rattus norvegicus (Rat)
样本类型:serum, plasma, cell culture supernates, tissue homogenates
检测范围:0.156 ng/mL-10 ng/mL
灵敏度:0.224 ng/mL
反应时间:1-5h
样本体积:50-100ul
检测波长:450 nm
研究领域:Cardiovascular
测定原理:quantitative
测定方法:Sandwich
精密度:
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
线性度:
To assess the linearity of the assay, samples were spiked with high concentrations of Rat TIMP-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
标准曲线:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
货期:3-5 working days
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