Human transforming growth factor β receptor 2 (TGF-βR2) ELISA kit

货号: IPD-E13669h

Price： ￥2500

48T 96T

数量：

大包装询价

产品描述：
人转化生长因子β受体2(TGF-βR2)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的TGFBR2含量。TGFBR2 是重要的靶点。它是转化生长因子-β（TGF-β）信号通路的关键受体。该通路参与细胞增殖、分化、凋亡等多种生理过程。异常激活或失活与肿瘤、纤维化等疾病相关。研究聚焦其信号传导机制及阻断策略，以开发新治疗手段。试剂盒检测范围为15.6 pg/mL-1000 pg/mL，灵敏度为3.9 pg/mL。适合用于疾病机制研究、药物干预效果评估或信号通路分析等科研场景，例如探究癌症微环境中受体表达变化或炎症模型中 TGF-β 通路活性调控本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：
TGFBR2; TGF-beta receptor type-2; TGFR-2; TGF-beta type II receptor; Transforming growth factor-beta receptor type II; TGF-beta receptor type II; TbetaR-II
缩写：
TGF-βR2
Uniprot No.：
P37173
种属：
Homo sapiens (Human)
样本类型：
serum, plasma, tissue homogenates
检测范围：
15.6 pg/mL-1000 pg/mL
灵敏度：
3.9 pg/mL
反应时间：
1-5h
样本体积：
50-100ul
检测波长：
450 nm
研究领域：
Cell Biology
测定原理：
quantitative
测定方法：
Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Intra-Assay PrecisionInter-Assay PrecisionSample123123n202020202020Mean(pg/ml)113.616109.567118.700121.407123.326117.167SD0.0320.0430.0380.0430.0490.052CV(%)4.1745.8734.8265.4026.0876.701

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human TGF-βRⅡ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

	Sample	Serum(n=4)
1:2	Average %	118
	Range %	108-120
1:4	Average %	111
	Range %	94-118
1:8	Average %	102
	Range %	94-105
1:16	Average %	94
	Range %	88-104

回收率：

The recovery of human TGF-βRⅡ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	102	94-115
EDTA plasma (n=4)	103	96-118

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.



pg/ml	OD1	OD2	Average	Corrected
1000	2.246	2.285	2.266	2.074
500	1.828	1.879	1.854	1.662
250	1.347	1.281	1.314	1.122
125	0.793	0.792	0.793	0.601
62.5	0.524	0.535	0.530	0.338
31.2	0.332	0.314	0.323	0.131
15.6	0.286	0.283	0.285	0.093
0	0.187	0.196	0.192