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Human Transforming growth factor-beta-induced protein ig-h3 (TGFBI/BIGH3) ELISA kit

  • 中文名： 人转化生长因子-β诱导蛋白IG - H3（TGFBI/BIGH3）ELISA Kit
货号: IPD-E16665h
Price： ￥2500
数量：
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产品详情

  • 产品描述：
    人转化生长因子-β诱导蛋白IG - H3（TGFBI/BIGH3）酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的TGFBI含量。TGFBI 是重要靶点。它在细胞生长、分化等生理过程有参与，其异常表达与肿瘤、眼部疾病等多种病理情况相关。研究机制主要聚焦于其信号通路，探索其与疾病发生、发展联系，以寻找潜在治疗策略，为开发新疗法提供方向。试剂盒检测范围为78 pg/mL-5000 pg/mL，灵敏度为19.5 pg/mL。适用于基础科研中探究该蛋白在纤维化疾病模型中的动态表达、肿瘤微环境基质重构研究，或用于评估药物干预对TGF-β通路下游效应分子的调控作用，为细胞信号转导、病理机制研究提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
  • 别名：
    RGD containing collagen associated protein ELISA Kit; AI181842 ELISA Kit; AI747162 ELISA Kit; Beta ig ELISA Kit; Beta ig h3 ELISA Kit; Beta ig-h3 ELISA Kit; BGH3_HUMAN ELISA Kit; Big h3 ELISA Kit; BIGH3 ELISA Kit; CDB1 ELISA Kit; CDG2 ELISA Kit; CDGG1 ELISA Kit; CSD ELISA Kit; CSD1 ELISA Kit; CSD2 ELISA Kit; CSD3 ELISA Kit; EBMD ELISA Kit; Kerato epithelin ELISA Kit; Kerato-epithelin ELISA Kit; LCD1 ELISA Kit; MGC150270 ELISA Kit; RGD CAP ELISA Kit; RGD-CAP ELISA Kit; RGD-containing collagen-associated protein ELISA Kit; TGFBI ELISA Kit; TGFBI transforming growth factor, beta induced, 68kDa ELISA Kit; Transforming growth factor beta induced protein ig h3 ELISA Kit; Transforming growth factor-beta-induced protein ig-h3 ELISA Kit
  • 缩写：
    TGFBI
  • Uniprot No.：
    Q15582
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    78 pg/mL-5000 pg/mL
  • 灵敏度：
    19.5 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Neuroscience
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：

    Intra-assay Precision (Precision within an assay): CV%<8%




    Three samples of known concentration were tested twenty times on one plate to assess.


    Inter-assay Precision (Precision between assays): CV%<10%




    Three samples of known concentration were tested in twenty assays to assess.



    　Intra-Assay PrecisionInter-Assay PrecisionSample123123n202020202020Mean(pg/ml)636.229580.524668.618598.802656.173586.342SD0.0460.0490.0390.0590.0680.058CV(%)4.2244.8323.4485.6796.0995.675







  • 线性度：

    To assess the linearity of the assay, samples were spiked with high concentrations of human TGFBI/BIGH3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.


    Sample

    Serum(n=4)


    1:200

    Average %

    106


    Range %

    94-110


    1:400

    Average %

    100


    Range %

    93-103


    1:800

    Average %

    94


    Range %

    81-98


    1:1600

    Average %

    104


    Range %

    95-108









  • 回收率：

    The recovery of human TGFBI/BIGH3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range


    Serum (n=5)

    92

    85-95


    EDTA plasma (n=4)

    99

    94-107









  • 标准曲线：

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.



















    pg/ml

    OD1

    OD2

    Average

    Corrected


    5000

    2.877

    2.762

    2.820

    2.722


    2500

    2.264

    2.333

    2.299

    2.301


    1250

    1.756

    1.684

    1.720

    1.622


    625

    1.099

    1.157

    1.128

    1.030


    312

    0.573

    0.585

    0.579

    0.381


    156

    0.299

    0.304

    0.302

    0.204


    78

    0.172

    0.168

    0.170

    0.072


    0

    0.096

    0.100

    0.098











  • 货期：
    3-5 working days

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