首页 / 产品 / ELISA试剂盒 / 其他ELISA试剂盒
产品描述：猪甲状腺素(T4)酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆样本中的T4含量。试剂盒检测范围为20 ng/mL-320 ng/mL，灵敏度为20 ng/mL。适用于农业科研中猪的生理周期监测、营养代谢研究、繁殖性能评估以及环境应激对内分泌系统影响的实验分析，为动物模型构建和基础医学研究提供可靠数据支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：T4
种属：Sus scrofa (Pig)
样本类型：serum, plasma
检测范围：20 ng/mL-320 ng/mL
灵敏度：20 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Competitive
精密度：
| Intra-assay Precision (Precision within an assay): CV%<15% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<15% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of pig T4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 88 | |||||
| Range % | 82-100 | ||||||
| 1:2 | Average % | 89 | |||||
| Range % | 83-98 | ||||||
| 1:4 | Average % | 95 | |||||
| Range % | 89-100 | ||||||
| 1:8 | Average % | 95 | |||||
| Range % | 90-100 | ||||||
回收率：
| The recovery of pig T4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 95 | 90-99 | |||||
| EDTA plasma (n=4) | 91 | 86-99 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| ng/mlOD1OD2Average 3200.1650.1690.167 1600.2790.2720.276 800.4950.4990.497 401.0271.0111.019 202.0522.0312.042 | |||||||
货期：3-5 working days
×
