首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠甲状腺素(T4)酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的T4含量。小鼠甲状腺素T4是甲状腺分泌的关键激素，主要参与调节新陈代谢、生长发育和神经系统功能。其作用机制涉及直接作用于破骨细胞和成骨细胞，影响骨钙代谢，并通过TSH受体调节骨形成和吸收。此外，T4还与季节性繁殖、肠道菌群和心脏功能等方面有关。试剂盒检测范围为20 ng/mL-320 ng/mL，灵敏度为20 ng/mL。常用于科研领域的小鼠模型甲状腺相关疾病研究、药物作用评估及代谢调控机制探索；适用于实验室对内分泌系统功能研究、药物干预效果评价以及代谢性疾病（如肥胖、糖尿病）模型的机制分析等场景本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：T4
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates
检测范围：20 ng/mL-320 ng/mL
灵敏度：20 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Competitive
精密度：
Intra-assay Precision (Precision within an assay): CV%<15%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<15%
Three samples of known concentration were tested in twenty assays to assess.
标准曲线：
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
本试剂盒所含材料：
A 96-well Assay plate -- The 96-well plate has been pre-coated with anti-mouse T4 antibody.
Standard 6 x 1 ml -- Dilute the standard at dilution series, read the OD values, and then draw a standard curve.
Biotin-conjugated T4 1 x 6 ml
HRP-avidin 1 x 6 ml -- HRP catalyzes the TMB to elicit a chromogenic reaction.
Wash Buffer (20 x concentrate) 1 x 15 ml -- Wash away unbound or free substances.
Substrate A 1 x 7 ml -- Mix with substrate B, and the TMB is catalyzed by HRP to develop color.
本试剂盒不含材料：
A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 600 nm to 630 nm.
An incubator that can provide stable incubation conditions up to 37°C±5°C.
Centrifuge
Vortex
Squirt bottle, manifold dispenser, or automated microplate washer
Absorbent paper for blotting the microtiter plate
货期：3-5 working days
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