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产品描述：山羊甲状腺素(T4)酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的T4含量。试剂盒检测范围为20 ng/mL-320 ng/mL，灵敏度为20 ng/mL。适用于科研领域中对山羊或其他偶蹄目动物甲状腺功能的动态监测，例如在营养代谢研究、环境内分泌干扰物评估、激素替代治疗模型构建中提供定量数据支持，亦可应用于畜牧学科研中饲料添加剂对甲状腺轴影响的药效学分析。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：T4
种属：Capra hircus (Goat)
样本类型：serum, plasma, tissue homogenates
检测范围：20 ng/mL-320 ng/mL
灵敏度：20 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Competitive
精密度：
| Intra-assay Precision (Precision within an assay): CV%<15% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<15% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of goat T4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 93 | |||||
| Range % | 86-101 | ||||||
| 1:2 | Average % | 86 | |||||
| Range % | 81-98 | ||||||
| 1:4 | Average % | 95 | |||||
| Range % | 90-100 | ||||||
| 1:8 | Average % | 95 | |||||
| Range % | 90-100 | ||||||
回收率：
| The recovery of goat T4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 97 | 92-110 | |||||
| EDTA plasma (n=4) | 95 | 90-105 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| ng/mlOD1OD2Average 3200.1630.1690.166 1600.2750.2810.278 800.4930.5020.498 401.0151.0291.022 202.0492.0682.059 | |||||||
货期：3-5 working days
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