首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠三碘甲状腺原氨酸(T3)酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆样本中的T3含量。靶点研究旨在识别疾病相关分子，作为药物干预目标。通过生物信息学、基因组学等技术，挖掘差异表达基因，构建蛋白质相互作用网络，寻找关键节点和基因簇，预测潜在治疗药物，为疾病治疗提供新思路。试剂盒检测范围为0.5 ng/mL-8 ng/mL，灵敏度为0.5 ng/mL。适用于科研领域中对甲状腺激素代谢机制、药物干预效果评估、疾病动物模型构建等研究需求，为体外实验提供稳定可靠的检测工具本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：T3
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, cell culture supernates, tissue homogenates
检测范围：0.5 ng/mL-8 ng/mL
灵敏度：0.5 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Competitive
精密度：
Intra-assay Precision (Precision within an assay): CV%<15%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<15%
Three samples of known concentration were tested in twenty assays to assess.
标准曲线：
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
本试剂盒所含材料：
A 96-well Assay plate --The 96-well plate has been pre-coated with an anti-human Ts antibody.
Standard(10 x concentrate) (6 x 1 ml) --Dilute the standard at dilution series, read the OD values, and then draw a standard curve.
Biotin-conjugated T3 (1 x 6 ml)
HRP-avidin (1 x 6 ml) --Catalyze the TMB, eliciting a chromogenic reaction.
Wash Buffer (20x concentrate) (1 x 15 ml) --Wash away unbound or free substances.
Substrate A (1x 7 ml) --Mix with Substrate B.
本试剂盒不含材料：
A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 600 nm - 630 nm.
An incubator that can provide stable incubation conditions up to 37°C±5°
Centrifuge
Vortex
Squirt bottle, manifold dispenser, or automated microplate washer
Absorbent paper for blotting the microtiter plate
货期：3-5 working days
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