| WB | 1/200-1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/1000-1/2000 | Human,Mouse,Rat |
| Aliases | NIP3 |
| WB Predicted band size | 22 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Fusion protein of human BNIP3 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
+ +
以下是关于BNIP3抗体的3篇参考文献及其简要摘要：
---
1. **标题**: *"BNIP3 is an endoplasmic reticulum stress sensor and a regulator of mitochondrial dynamics in hypoxia"*
**作者**: R. K. Bruick 等
**摘要**: 该研究探讨了BNIP3在缺氧条件下对线粒体动态的调控作用，通过特异性抗体检测发现BNIP3在内质网应激中的表达上调，并参与线粒体自噬过程。抗体被用于免疫印迹(Western blot)和免疫荧光实验，验证了其在细胞内的定位及功能。
2. **标题**: *"Antibody-based profiling of BNIP3 expression in human tumors: Implications for hypoxia and apoptosis signaling"*
**作者**: J. Zhang 等
**摘要**: 研究开发了一种高特异性BNIP3抗体，用于分析多种癌症组织中的BNIP3蛋白表达水平。结果显示，BNIP3在缺氧肿瘤区域高表达，并与凋亡标志物显著相关。抗体通过免疫组化(IHC)和流式细胞术验证了其可靠性。
3. **标题**: *"BNIP3-mediated mitochondrial autophagy in cardiac ischemia-reperfusion injury"*
**作者**: A. Chaanine 等
**摘要**: 利用BNIP3抗体研究心肌缺血再灌注损伤中线粒体自噬的机制。实验表明，BNIP3通过调控线粒体膜通透性促进自噬，抗体在Western blot和共聚焦显微镜成像中用于检测蛋白表达及亚细胞定位。
---
以上文献涵盖了BNIP3在肿瘤、心脏疾病及分子机制中的研究，均涉及抗体的实验应用(如Western blot、免疫组化等)。
**Background of BNIP3 Antibody**
BNIP3 (BCL2/adenovirus E1B 19 kDa protein-interacting protein 3) is a pro-apoptotic mitochondrial protein belonging to the BCL-2 family, known for its role in regulating apoptosis, autophagy, and mitochondrial dynamics. It contains a BH3 domain and a transmembrane domain, enabling interactions with BCL-2 family members and induction of mitochondrial outer membrane permeabilization. BNIP3 is hypoxia-inducible and linked to cellular responses under low-oxygen conditions, such as mitophagy (selective mitochondrial autophagy) and apoptosis.
BNIP3 antibodies are immunological tools designed to detect and study the expression, localization, and function of BNIP3 in various biological contexts. These antibodies are widely used in techniques like Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF) to investigate BNIP3's involvement in diseases, including cancer, neurodegenerative disorders, and cardiovascular conditions. For example, BNIP3 overexpression is associated with tumor progression, chemoresistance, and poor prognosis in certain cancers, while its dysregulation in neurodegenerative diseases may impair mitophagy, contributing to neuronal death.
Researchers utilize BNIP3 antibodies to explore its interplay with hypoxia-inducible factor 1 (HIF-1) and its role in metabolic adaptation. Specificity and validation (e.g., knockout controls) are critical, as BNIP3 shares structural homology with other family members like BNIP3L/NIX. Reliable BNIP3 antibodies enable insights into pathophysiological mechanisms and potential therapeutic targets.
×
