Recombinant Human NAIF1 protein

中文名： 核细胞凋亡诱导因子1(NAIF1)重组蛋白
别名： NAIF1;C9orf90;Nuclear apoptosis-inducing factor 1

货号: PA1000-2078

Price： ￥询价

20μg 50μg 100μg ＞100μg

数量：

大包装询价

产品详情

纯度	>90%SDS-PAGE.
种属	Human
靶点	NAIF1
Uniprot No	Q69YI7
内毒素	< 0.01EU/μg
表达宿主	E.coli
表达区间	1-327aa
氨基酸序列	MGSSHHHHHHSSGLVPRGSHMGSMAVPAKKRKMNFSEREVEIIVEELELK KHLLVNHFNAGVPLAAKSAAWHGILRRVNAVATCRRELPEVKKKWSDLKT EVRRKVAQVRAAVEGGEAPGPTEEDGAGGPGTGGGSGGGGPAVAPVLLTP MQQRICNLLGEATIISLPSTTEIHPVALGPSATAAAATVTLTQIPTETTY HTLEEGVVEYCTAEAPPPLPPETPVDMMAQHADTSVKPQALKSRIALNSA KLIQEQRVTNLHVKEIAQHLEQQNDLLQMIRRSQEVQACAQERQAQAMEG TQAALSVLIQVLRPMIKDFRRYLQSNTANPAPASDPGQVAQNGQPDSIIQ
预测分子量	38 kDa
蛋白标签	His tag N-Terminus
缓冲液	PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
稳定性 & 储存条件	Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
复溶	Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

参考文献

以下是关于NAIF1重组蛋白的3篇代表性文献及其摘要内容：

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1. **文献名称**: *"Cloning and functional characterization of NAIF1 reveals its pro-apoptotic activity"*

**作者**: Zhang Y. et al. (2015)

**摘要**: 该研究首次报道了人源NAIF1重组蛋白的克隆与表达，发现其通过激活caspase通路诱导HeLa细胞凋亡，提示其在肿瘤抑制中的潜在作用。

2. **文献名称**: *"Crystal structure of NAIF1 reveals a conserved NLR family scaffold"*

**作者**: Chen L. et al. (2018)

**摘要**: 通过X射线晶体学解析了重组NAIF1蛋白的三维结构，发现其具有典型的NLR家族核苷酸结合结构域，为研究其与炎症小体的相互作用提供结构基础。

3. **文献名称**: *"NAIF1 regulates innate immunity by interacting with mitochondrial antiviral signaling protein"*

**作者**: Li X. et al. (2020)

**摘要**: 研究表明，重组NAIF1蛋白通过与MAVS蛋白结合，增强I型干扰素产生，揭示了其在抗病毒天然免疫反应中的调控机制。

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注：以上文献信息为示例性质，实际研究中建议通过PubMed或Web of Science以“NAIF1”或“NLR family apoptosis inhibitory protein 1”为关键词检索最新文献。

背景信息

**Background of NAIF1 Recombinant Protein**

NAIF1 (Nuclear Apoptosis-Inducing Factor 1) is a protein implicated in cellular processes such as apoptosis, DNA repair, and immune regulation. Initially identified for its role in modulating programmed cell death, NAIF1 interacts with key apoptotic factors, including caspases and Bcl-2 family proteins, influencing mitochondrial membrane integrity and cytochrome c release. Recent studies highlight its broader involvement in innate immunity, particularly in antiviral responses. NAIF1 is reported to regulate type I interferon (IFN) signaling by interacting with nucleic acid sensors like RIG-I and MDA5. thereby modulating antiviral defense mechanisms.

Recombinant NAIF1 protein is engineered using heterologous expression systems (e.g., *E. coli* or mammalian cells*) to produce purified, biologically active forms for research and therapeutic applications. Its recombinant production enables detailed structural and functional studies, including mapping interaction domains and elucidating signaling pathways. NAIF1's role in apoptosis and immune regulation positions it as a potential therapeutic target for diseases like cancer, viral infections, and autoimmune disorders. For instance, modulating NAIF1 activity could enhance antiviral responses or sensitize cancer cells to apoptosis.

Despite progress, gaps remain in understanding its post-translational modifications, tissue-specific functions, and cross-talk with other immune pathways. Current research focuses on leveraging recombinant NAIF1 to develop targeted therapies and diagnostic tools, emphasizing its dual role in cell death and immunity. Its therapeutic potential, combined with advances in protein engineering, underscores NAIF1's growing significance in biomedical research.