Human sex hormone-binding globulin,SHBG ELISA Kit

中文名： 人性激素结合球蛋白(SHBG)ELISA Kit

货号: IPD-E08232h

Price： ￥2500

48T 96T

数量：

大包装询价

产品详情

产品描述：
人性激素结合球蛋白(SHBG)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的SHBG含量。SHBG即性激素结合球蛋白，它能特异性结合性激素，在性激素的运输、代谢及生物活性调节中起关键作用。研究主要聚焦于其在激素相关疾病中的机制，如通过影响性激素水平参与疾病发生发展，为疾病诊断、治疗及药物研发提供新思路。试剂盒检测范围为1.56 pmol/L- 100 pmol/L，灵敏度为0.39 pmol/L。适用于科研领域探究多囊卵巢综合征、性腺功能异常、肥胖相关激素紊乱等疾病的分子机制，也可用于评估激素替代疗法或药物干预对性激素代谢的影响。支持研究者高效获取人源生物样本中SHBG的动态变化数据，为生殖内分泌基础研究及转化医学提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：
ABP ELISA Kit; Androgen binding protein ELISA Kit; SBP ELISA Kit; Sex hormone-binding globulin ELISA Kit; Sex steroid binding protein ELISA Kit; Sex steroid-binding protein ELISA Kit; SHBG ELISA Kit; SHBG_HUMAN ELISA Kit; TeBG ELISA Kit; Testis specific androgen binding protein ELISA Kit; Testis-specific androgen-binding protein ELISA Kit; Testosterone binding beta globulin ELISA Kit; Testosterone estradiol binding globulin ELISA Kit; Testosterone estrogen binding globulin ELISA Kit; Testosterone-estradiol-binding globulin ELISA Kit; Testosterone-estrogen-binding globulin ELISA Kit
缩写：
SHBG
Uniprot No.：
P04278
种属：
Homo sapiens (Human)
样本类型：
serum, plasma, tissue homogenates
检测范围：
1.56 pmol/L- 100 pmol/L
灵敏度：
0.39 pmol/L
反应时间：
1-5h
样本体积：
50-100ul
检测波长：
450 nm
研究领域：
Signal Transduction
测定原理：
quantitative
测定方法：
Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

　Intra-Assay PrecisionInter-Assay PrecisionSample123123n202020202020Mean(pmol/L)12.02412.17212.19712.04912.14712.271SD0.0390.0490.0580.0480.0590.065CV(%)4.4425.5436.5545.4616.6827.320

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human SHBG in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

Sample

Serum(n=4)

1:1000

Average %

Range %

91-100

1:2000

Average %

Range %

88-97

1:4000

Average %

Range %

94-103

1:8000

Average %

Range %

80-88

回收率：

The recovery of human SHBG spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	89-98
EDTA plasma (n=4)	90	85-94

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.













pmol/L	OD1	OD2	Average	Corrected
100	2.132	2.092	2.112	1.912
50	1.701	1.699	1.700	1.519
25	1.298	1.304	1.301	1.124
12.5	0.845	0.850	0.848	0.670
6.25	0.628	0.633	0.631	0.453
3.12	0.388	0.395	0.391	0.215
1.56	0.279	0.274	0.276	0.094
0	0.178	0.181	0.180