Rat plasminogen activator inhibitor 1,PAI1 ELISA Kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

货号: IPD-E07948r

Price： ￥2500

48T 96T

数量：

大包装询价

产品描述：
大鼠纤溶酶原激活物抑制因子1(PAI1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的SERPINE1含量。SERPINE1 即丝氨酸蛋白酶抑制剂 E1，也叫纤溶酶原激活物抑制剂 -1（PAI-1）。其与多种疾病如心血管病、肿瘤等密切相关。研究机制上，它主要通过抑制纤溶酶原激活物来调控纤溶系统，还参与细胞迁移、黏附等过程并影响疾病发生发展。试剂盒检测范围为78 pg/ml-5000 pg/ml，灵敏度为19.5 pg/ml。适用于心血管疾病、代谢综合征或器官纤维化等科研领域的大鼠模型研究，例如通过检测不同干预条件下PAI1在血液循环或组织中的浓度变化，探索其在血栓调节、细胞外基质代谢中的作用机制。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：
Serpine1 ELISA Kit; Pai1 ELISA Kit; Planh1 ELISA Kit; Plasminogen activator inhibitor 1 ELISA Kit; PAI ELISA Kit; PAI-1 ELISA Kit; Endothelial plasminogen activator inhibitor ELISA Kit; Serpin E1 ELISA Kit
缩写：
SERPINE1
Uniprot No.：
P20961
种属：
Rattus norvegicus (Rat)
样本类型：
serum, plasma, tissue homogenates
检测范围：
78 pg/ml-5000 pg/ml
灵敏度：
19.5 pg/ml
反应时间：
1-5h
样本体积：
50-100ul
检测波长：
450 nm
研究领域：
Cell Biology
测定原理：
quantitative
测定方法：
Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat PAI1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

	Sample	Serum(n=4)
1:1	Average %	93
	Range %	86-98
1:2	Average %	93
	Range %	88-100
1:4	Average %	88
	Range %	80-92
1:8	Average %	90
	Range %	86-95

回收率：

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.


pg/ml	OD1	OD2	Average	Corrected
5000	2.630	2.634	2.632	2.463
2500	1.874	1.767	1.820	1.651
1250	1.153	1.128	1.140	0.971
625	0.679	0.649	0.664	0.495
312	0.506	0.521	0.513	0.344
156	0.318	0.299	0.308	0.139
78	0.258	0.264	0.261	0.092
0	0.173	0.165	0.169