Human E-Selectin ELISA kit

中文名： 人E选择素(E-Selectin/CD62E)ELISA kit

货号: IPD-E04540h

Price： ￥2500

48T 96T

数量：

大包装询价

产品详情

产品描述：
人E选择素(E-Selectin/CD62E)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的SELE含量。SELE 即选择素 E，主要表达于活化的血管内皮细胞。其背景在于在炎症反应中起关键作用。研究机制方面，它能介导白细胞与内皮细胞的初始黏附，促使白细胞滚动，利于白细胞迁移至炎症部位，在炎症、肿瘤转移等病理过程研究中备受关注。试剂盒检测范围为0.312 ng/mL-20 ng/mL，灵敏度为0.078 ng/mL。适用于体外细胞模型、动物实验或临床前研究中内皮细胞激活状态的动态监测，也可用于炎症性疾病模型的生物标志物分析或药物干预效果的评估，支持科研实验室开展多样本、多批次的E选择素表达水平检测需求。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：
SELE; ELAM1; E-selectin; CD62 antigen-like family member E; Endothelial leukocyte adhesion molecule 1; ELAM-1; Leukocyte-endothelial cell adhesion molecule 2; LECAM2; CD antigen CD62E
缩写：
SELE
Uniprot No.：
P16581
种属：
Homo sapiens (Human)
样本类型：
serum, plasma, tissue homogenates
检测范围：
0.312 ng/mL-20 ng/mL
灵敏度：
0.078 ng/mL
反应时间：
1-5h
样本体积：
50-100ul
检测波长：
450 nm
研究领域：
Immunology
测定原理：
quantitative
测定方法：
Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

　Intra-Assay PrecisionInter-Assay PrecisionSample123123n202020202020Mean(ng/ml)2.3822.6292.4632.5642.5922.546SD0.0410.0430.0360.0490.0520.047CV(%)5.765.614.936.526.866.28

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human E-selectin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

Sample

Serum(n=4)

1:1

Average %

Range %

95-110

1:2

Average %

Range %

91-103

1:4

Average %

Range %

82-92

1:8

Average %

Range %

89-98

回收率：

The recovery of human E-selectin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	89-99
Heparin plasma (n=4)	98	90-101

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.












ng/ml	OD1	OD2	Average	Corrected
20	2.370	2.274	2.322	2.154
10	1.859	1.779	1.819	1.651
5	1.229	1.209	1.219	1.051
2.5	0.740	0.734	0.737	0.569
1.25	0.437	0.431	0.434	0.266
0.625	0.347	0.339	0.343	0.175
0.312	0.207	0.204	0.206	0.038
0	0.168	0.167	0.168