首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠抵抗素(Resistin)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆样本中的RETN含量。RETN（抵抗素）是一种由脂肪组织分泌的激素，与胰岛素抵抗和炎症反应密切相关。研究显示，RETN基因多态性与多种疾病如多囊卵巢综合征、2型糖尿病和心血管疾病等存在关联。目前，RETN在代谢和生殖健康方面的作用成为研究热点，其具体机制尚在探索中。试剂盒检测范围为62.5 pg/mL-4000 pg/mL，灵敏度为41.036 pg/mL。适用于探索小鼠模型中Resistin在代谢调控、炎症通路中的作用机制，例如通过检测肥胖模型血清Resistin水平变化，或分析脂肪组织匀浆中蛋白表达差异。其宽泛的线性范围与多类型样本兼容性，可支持细胞实验、动物模型及离体组织研究，为糖代谢相关科研提供稳定、可重复的数据支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Retn ELISA Kit; Fizz3 ELISA Kit; Resistin ELISA Kit; Adipose tissue-specific secretory factor ELISA Kit; ADSF ELISA Kit; Adipose-specific cysteine-rich secreted protein A12-alpha ELISA Kit; Cysteine-rich secreted protein FIZZ3 ELISA Kit
缩写：RETN
Uniprot No.：Q99P87
种属：Mus musculus (Mouse)
样本类型：serum, plasma, cell culture supernates, tissue homogenates
检测范围：62.5 pg/mL-4000 pg/mL
灵敏度：41.036 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cardiovascular
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse resistin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:100 | Average % | 96 | ||||
| Range % | 91-103 | |||||
| 1:200 | Average % | 85 | ||||
| Range % | 80-90 | |||||
| 1:400 | Average % | 93 | ||||
| Range % | 89-98 | |||||
| 1:800 | Average % | 100 | ||||
| Range % | 94-105 | |||||
回收率：
| The recovery of mouse resistin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 91 | 85-96 | ||||
| EDTA plasma (n=4) | 94 | 90-98 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected 40002.5242.4852.5052.318 20001.9172.0071.9621.775 10001.2941.3521.3231.136 5000.8930.8490.8710.684 2500.6270.6010.6140.427 1250.4210.4470.4340.247 62.50.2970.2850.2910.104 00.1900.1830.187 | ||||||
货期：3-5 working days
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