首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人抵抗素(Resistin)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的RETN含量。RETN即抵抗素，是脂肪组织分泌的一种富含半胱氨酸的蛋白质。它与胰岛素抵抗、肥胖、糖尿病等密切相关。研究机制上，其可能通过影响胰岛素信号通路、调节糖脂代谢等，在代谢性疾病的发生发展中发挥重要作用，目前是相关领域研究热点。试剂盒检测范围为0.312 ng/mL-20 ng/mL，灵敏度为0.078 ng/mL。适用于体外研究领域中对Resistin表达水平的定量分析，例如探究代谢综合征发病机制、评估药物干预对脂肪因子调控的影响，或进行肥胖相关疾病的分子生物学研究。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Adipose tissue specific secretory factor ELISA Kit; Adipose tissue-specific secretory factor ELISA Kit; ADSF ELISA Kit; C/EBP epsilon regulated myeloid specific secreted cysteine rich protein ELISA Kit; C/EBP epsilon regulated myeloid specific secreted cysteine rich protein precursor 1 ELISA Kit; C/EBP-epsilon-regulated myeloid-specific secreted cysteine-rich protein ELISA Kit; CG5403 ELISA Kit; Cysteine rich secreted protein A12 alpha like 2 ELISA Kit; Cysteine rich secreted protein FIZZ3 ELISA Kit; Cysteine-rich secreted protein A12-alpha-like 2 ELISA Kit; Cysteine-rich secreted protein FIZZ3 ELISA Kit; dri ELISA Kit; FIZZ 3 ELISA Kit; FIZZ3 ELISA Kit; Found in inflammatory zone 3 ELISA Kit; HXCP 1 ELISA Kit; HXCP1 ELISA Kit; MGC126603 ELISA Kit; MGC126609 ELISA Kit; PRO1199 ELISA Kit; Resistin ELISA Kit; Resistin delta2 ELISA Kit; RETN 1 ELISA Kit; RETN ELISA Kit; RETN_HUMAN ELISA Kit; RETN1 ELISA Kit; RSTN ELISA Kit; UNQ407 ELISA Kit; XCP 1 ELISA Kit; XCP1 ELISA Kit
缩写：RETN
Uniprot No.：Q9HD89
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：0.312 ng/mL-20 ng/mL
灵敏度：0.078 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cardiovascular
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human resistin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 97 | ||||
| Range % | 92-103 | |||||
| 1:2 | Average % | 94 | ||||
| Range % | 91-98 | |||||
| 1:4 | Average % | 102 | ||||
| Range % | 98-106 | |||||
| 1:8 | Average % | 86 | ||||
| Range % | 80-92 | |||||
回收率：
| The recovery of human resistin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 97 | 90-104 | ||||
| EDTA plasma (n=4) | 92 | 88-97 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected 202.2852.3302.3082.204 101.3501.4271.3891.285 50.7010.7330.7170.613 2.50.4000.4240.4120.308 1.250.2560.2680.2620.158 0.6250.1780.1690.1740.070 0.3120.1320.1290.1310.027 00.1050.1020.104 | ||||||
货期：3-5 working days
×
