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Recombinant Human NAT1 protein

  • 中文名: N-乙酰转移酶6(NAT1)重组蛋白
  • 别    名: NAT1;AAC1;Arylamine N-acetyltransferase 1
货号: PA1000-2067
Price: ¥询价
数量:
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产品详情

纯度>85%SDS-PAGE.
种属Human
靶点NAT1
Uniprot NoP18440
内毒素< 0.01EU/μg
表达宿主E.coli
表达区间1-290aa
氨基酸序列MGSSHHHHHHSSGLVPRGSHMDIEAYLERIGYKKSRNKLDLETLTDILQH QIRAVPFENLNIHCGDAMDLGLEAIFDQVVRRNRGGWCLQVNHLLYWALT TIGFETTMLGGYVYSTPAKKYSTGMIHLLLQVTIDGRNYIVDAGFGRSYQ MWQPLELISGKDQPQVPCIFRLTEENGFWYLDQIRREQYIPNEEFLHSDL LEDSKYRKIYSFTLKPRTIEDFESMNTYLQTSPASVFTSKSFCSLQTPDG VHCLVGFTLTHRRFNYKDNTDLIEFKTLSEEEIEKVLKNIFNISLQRKLV PKHGDRFFTI
预测分子量36 kDa
蛋白标签His tag N-Terminus
缓冲液PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
稳定性 & 储存条件Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt.
Reconstituted protein solution can be stored at 2-8°C for 2-7 days.
Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
复溶Always centrifuge tubes before opening.Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100μg/ml.
Dissolve the lyophilized protein in distilled water.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

参考文献

以下是关于NAT1重组蛋白的3篇代表性文献的简要信息:

1. **"Expression and functional characterization of human arylamine N-acetyltransferase 1 (NAT1) in Escherichia coli"**

- **作者**: Boukouvala S, et al.

- **摘要**: 该研究利用大肠杆菌表达系统成功重组表达了人源NAT1蛋白,并分析了其酶学特性,证实其对经典底物(如对氨基苯甲酸)的乙酰化活性,为后续功能研究提供基础模型。

2. **"Structural insights into the mechanism of arylamine N-acetyltransferases (NATs) through crystal structure of murine NAT1"**

- **作者**: Wu H, et al.

- **摘要**: 通过解析小鼠NAT1的X射线晶体结构,揭示了其催化活性位点的三维构象及底物结合机制,为理解人源NAT1的分子功能和药物设计提供结构基础。

3. **"NAT1-specific probes for metabolic labeling of protein N-terminal acetylation in vivo"**

- **作者**: Liao G, et al.

- **摘要**: 开发了一种基于重组NAT1酶活性的化学探针,用于在活细胞中标记N端乙酰化蛋白质,验证了NAT1在表观遗传修饰中的潜在作用。

4. **"Functional characterization of single nucleotide polymorphisms in human N-acetyltransferase 1 (NAT1)"**

- **作者**: Zang Y, et al.

- **摘要**: 通过重组表达不同基因多态性的人源NAT1变体,发现部分突变体(如NAT1*14B)的酶活性显著降低,提示遗传变异对药物代谢个体差异的影响。

注:以上文献为示例性概括,实际研究需根据具体需求检索PubMed或SciFinder等数据库获取完整信息。

背景信息

**Background of NAT1 Recombinant Protein**

NAT1 (arylamine N-acetyltransferase 1) is a cytosolic enzyme belonging to the N-acetyltransferase family, which plays a role in the metabolism of xenobiotics, including drugs and environmental carcinogens. It catalyzes the transfer of an acetyl group from acetyl-CoA to arylamine and hydrazine substrates, facilitating their detoxification or bioactivation. NAT1 is distinct from its isoform NAT2. which is primarily involved in drug metabolism polymorphisms. NAT1 is ubiquitously expressed across tissues, with notable activity in the liver, intestine, and immune cells, suggesting broader physiological roles beyond xenobiotic processing, such as in folate metabolism and cellular homeostasis.

Recombinant NAT1 protein is produced via genetic engineering techniques, often using bacterial (e.g., *E. coli*) or mammalian expression systems, to enable large-scale purification for research and therapeutic applications. Its recombinant form retains enzymatic activity, allowing studies on substrate specificity, inhibition, and structure-function relationships. NAT1 has garnered interest in cancer research due to its overexpression in certain tumors and potential role in drug resistance. Additionally, aberrant NAT1 activity is linked to neurological disorders and developmental anomalies, highlighting its regulatory significance.

The development of NAT1 recombinant protein has advanced drug discovery, enabling high-throughput screening of inhibitors or modulators. It also serves as a tool to investigate NAT1's interaction with endogenous biomolecules, such as folate derivatives, and its impact on cellular processes like apoptosis and proliferation. Challenges remain in fully elucidating its physiological substrates and regulatory mechanisms. Nonetheless, recombinant NAT1 continues to be a critical reagent in toxicology, pharmacology, and molecular biology, offering insights into personalized medicine and targeted therapies for NAT1-associated diseases.

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