首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人视黄醇结合蛋白4(RBP-4)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的RBP4含量。RBP4（视黄醇结合蛋白4）是一种由肝细胞合成的蛋白质，主要功能是结合并转运维生素A。其研究机制涉及在脂代谢、胰岛素抵抗、动脉硬化、高血压和心力衰竭等心血管疾病中的作用，与这些疾病的病理生理过程密切相关。试剂盒检测范围为15.6 ng/mL-1000 ng/mL，灵敏度为3.9 ng/mL。适用于肥胖、2型糖尿病、非酒精性脂肪肝等代谢性疾病的基础研究，或营养干预实验中RBP - 4动态变化的追踪，为体外样本的蛋白质组学研究提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：OTTHUMP00000020114 ELISA Kit; OTTHUMP00000020115 ELISA Kit; OTTHUMP00000020116 ELISA Kit; Plasma retinol binding protein 4 ELISA Kit; Plasma retinol-binding protein ELISA Kit; Plasma retinol-binding protein(1-176) ELISA Kit; PRBP ELISA Kit; PRO2222 ELISA Kit; RBP ELISA Kit; RBP4 ELISA Kit; RDCCAS ELISA Kit; RET4_HUMAN ELISA Kit; Retinol binding protein 4 ELISA Kit; Retinol binding protein 4 interstitial ELISA Kit; Retinol binding protein 4 plasma ELISA Kit
缩写：RBP4
Uniprot No.：P02753
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：15.6 ng/mL-1000 ng/mL
灵敏度：3.9 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human RBP-4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:100 | Average % | 92 | ||||
| Range % | 87-98 | |||||
| 1:200 | Average % | 98 | ||||
| Range % | 93-104 | |||||
| 1:400 | Average % | 84 | ||||
| Range % | 82-88 | |||||
| 1:800 | Average % | 90 | ||||
| Range % | 85-97 | |||||
回收率：
| The recovery of human RBP-4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 89-99 | ||||
| EDTA plasma (n=4) | 96 | 90-102 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected 10002.6342.7022.6682.566 5002.2342.2172.2262.124 2501.4961.5271.5121.410 1250.8560.8790.8680.766 62.50.4460.4680.4570.355 31.20.2670.2850.2760.174 15.60.2060.2120.2090.107 00.1050.0990.102 | ||||||
货期：3-5 working days
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