首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人趋化素酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的RARRES2含量。RARRES2是一种调节脂肪生成和脂肪细胞脂质代谢的脂肪因子，与癌症发生发展有关，具有肿瘤抑制作用。其研究机制主要涉及通过调节脂质代谢重编程介导三阴性乳腺癌脑转移的发展，并在乳腺癌脑转移诊疗中具有潜在应用价值。试剂盒检测范围为31.2 ng/ml-2000 ng/ml，灵敏度为7.8 ng/mL。该产品适用于基础科研中探究Chemerin在代谢性疾病动物模型、细胞实验或组织样本中的表达调控，如评估肥胖、糖尿病等病理状态下脂肪组织分泌功能变化，或炎症微环境中趋化因子网络的动态响应。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：HP10433 ELISA Kit; RAR responsive protein TIG2 ELISA Kit; RAR-responsive protein TIG2 ELISA Kit; RARR2_HUMAN ELISA Kit; RARRES2 ELISA Kit; Retinoic acid receptor responder (tazarotene induced) 2 ELISA Kit; Retinoic acid receptor responder 2 ELISA Kit; Retinoic acid receptor responder protein 2 ELISA Kit; Tazarotene induced gene 2 protein ELISA Kit; Tazarotene-induced gene 2 protein ELISA Kit; TIG2 ELISA Kit
缩写：RARRES2
Uniprot No.：Q99969
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：31.2 ng/ml-2000 ng/ml
灵敏度：7.8 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cardiovascular
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human chemerin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||
| Sample | Serum(n=4) | ||
| 1:1 | Average % | 96 | |
| Range % | 90-103 | ||
| 1:2 | Average % | 97 | |
| Range % | 91-105 | ||
| 1:4 | Average % | 95 | |
| Range % | 92-100 | ||
| 1:8 | Average % | 93 | |
| Range % | 85-99 |
回收率：
| The recovery of human chemerin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 88-99 | ||||
| EDTA plasma (n=4) | 96 | 90-101 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected 20002.4922.4522.4722.295 10001.9671.9761.9721.795 5001.3141.2931.3041.127 2500.7680.7920.7800.603 1250.4720.4520.4620.285 62.50.3840.3670.3760.199 31.20.2980.2950.2970.120 00.1760.1770.177 | ||||||
货期：3-5 working days
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