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Mouse Progesterone,PROG ELISA Kit

  • 中文名: 小鼠孕激素/孕酮(PROG)ELISA Kit
货号: IPD-E05104m
Price: ¥2500
数量:
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产品详情

  • 产品描述:
    小鼠孕激素/孕酮(PROG)酶联免疫试剂盒为竞争法ELISA试剂盒,定量检测血清、血浆样本中的PROG含量。小鼠孕激素/孕酮(PROG)在妊娠和生殖过程中发挥关键作用,调控子宫内膜和胎盘发育,维持妊娠稳定。其作用机制涉及调节基因表达、影响细胞增殖和凋亡,并与孕酮受体结合,参与激素信号传导。试剂盒检测范围为0.3 ng/mL-10 ng/mL,灵敏度为0.2 ng/mL。适用于生殖生物学、内分泌调控机制研究及药物/化合物对生殖系统影响的评估,例如探究妊娠动物模型激素动态、卵巢功能异常研究或激素替代治疗相关实验。支持科研人员高效开展小鼠生殖健康、代谢调控等领域的基础研究。本品仅用于科研,不用于临床诊断,产品具体参数及操作步骤详见产品说明书。
  • 缩写:
    PROG
  • 种属:
    Mus musculus (Mouse)
  • 样本类型:
    serum, plasma
  • 检测范围:
    0.3 ng/mL-10 ng/mL
  • 灵敏度:
    0.2 ng/mL
  • 反应时间:
    1-5h
  • 样本体积:
    50-100ul
  • 检测波长:
    450 nm
  • 研究领域:
    Signal Transduction
  • 测定原理:
    quantitative
  • 测定方法:
    Competitive
  • 精密度:

    Intra-assay Precision (Precision within an assay): CV%<15%




    Three samples of known concentration were tested twenty times on one plate to assess.


    Inter-assay Precision (Precision between assays): CV%<15%




    Three samples of known concentration were tested in twenty assays to assess.



  • 线性度:

    To assess the linearity of the assay, samples were spiked with high concentrations of mouse PROG in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.


    Sample

    Serum(n=4)


    1:1

    Average %

    96


    Range %

    92-104


    1:2

    Average %

    93


    Range %

    91-97


    1:4

    Average %

    102


    Range %

    98-106


    1:8

    Average %

    87


    Range %

    82-94


  • 回收率:

    The recovery of mouse PROG spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range


    Serum (n=5)

    94

    90-97


    EDTA plasma (n=4)

    89

    85-94


  • 标准曲线:

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.














    ng/ml

    OD1

    OD2

    Average



    10

    0.175

    0.189

    0.182



    5

    0.452

    0.456

    0.454



    2.5

    0.759

    0.783

    0.771



    0.86

    1.175

    1.251

    1.213



    0.3

    1.647

    1.685

    1.666



    0

    2.085

    2.130

    2.107




  • 货期:
    3-5 working days

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