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Human Progesterone,PROG ELISA kit

  • 中文名： 人孕激素/孕酮(PROG)ELISA Kit
货号: IPD-E07283h
Price： ￥2500
数量：
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产品详情

  • 产品描述：
    人孕激素/孕酮(PROG)酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆.样本中的PROG含量。试剂盒检测范围为1 ng/ml-70 ng/ml，灵敏度为0.2 ng/ml。本试剂盒适用于科研领域中对哺乳动物生殖生理机制、内分泌紊乱相关疾病模型或药物干预效果的研究，可精准分析生物样本中孕酮的动态变化，为生殖医学、动物科学及药理学研究提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
  • 缩写：
    PROG
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma.
  • 检测范围：
    1 ng/ml-70 ng/ml.
  • 灵敏度：
    0.2 ng/ml.
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Signal Transduction
  • 测定原理：
    quantitative
  • 测定方法：
    Competitive
  • 精密度：

    Intra-assay Precision (Precision within an assay): CV%<15%




    Three samples of known concentration were tested twenty times on one plate to assess.


    Inter-assay Precision (Precision between assays): CV%<15%




    Three samples of known concentration were tested in twenty assays to assess.



  • 线性度：

    To assess the linearity of the assay, samples were spiked with high concentrations of human PROG in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.


    Sample

    Serum(n=4)


    1:1

    Average %

    92


    Range %

    87-98


    1:2

    Average %

    95


    Range %

    89-105


    1:4

    Average %

    90


    Range %

    83-102


    1:8

    Average %

    95


    Range %

    90-103


  • 回收率：

    The recovery of human PROG spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range


    Serum (n=5)

    95

    89-98


    EDTA plasma (n=4)

    94

    90-100


  • 标准曲线：

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.







    ng/ml

    OD1

    OD2

    Average



    0

    2.356

    2.380

    2.368



    1

    1.851

    1.836

    1.843



    3

    1.437

    1.481

    1.459



    10

    0.847

    0.886

    0.867



    30

    0.507

    0.523

    0.515



    70

    0.302

    0.316

    0.309




  • 货期：
    3-5 working days

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