Human Tissue-type Plasiminogen Actilyse,t-PA ELISA Kit

中文名： 人组织型纤溶酶原激活剂(t-PA)ELISA Kit

货号: IPD-E07916h

Price： ￥2500

48T 96T

数量：

大包装询价

产品详情

产品描述：
人组织型纤溶酶原激活剂(t-PA)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆.样本中的PLAT含量。PLAT即组织型纤溶酶原激活剂，是一种丝氨酸蛋白酶。它在体内参与纤维蛋白溶解过程，可特异性激活纤溶酶原转化为纤溶酶，以溶解血栓。其研究机制聚焦于激活机制、与抑制剂互作，在血栓性疾病治疗上有重要研究价值。试剂盒检测范围为0.156 ng/ml-10 ng/ml，灵敏度为0.039 ng/ml。适用于体外研究场景，如药物开发中溶栓药物效价评估、心血管疾病模型中的纤溶功能研究，或组织工程中t-PA调控机制的探索，为科研人员提供标准化、高重复性的检测方案。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：
Alteplase ELISA Kit; DKFZp686I03148 ELISA Kit; Plasminogen activator tissue ELISA Kit; Plasminogen activator tissue type ELISA Kit; PLAT ELISA Kit; Reteplase ELISA Kit; t PA ELISA Kit; T Plasminogen Activator ELISA Kit; t-PA ELISA Kit; T-plasminogen activator ELISA Kit; Tissue plasminogen activator (t PA) ELISA Kit; Tissue type plasminogen activator ELISA Kit; Tissue-type plasminogen activator chain B ELISA Kit; tPA ELISA Kit; TPA_HUMAN ELISA Kit; TPA1 ELISA Kit
缩写：
PLAT
Uniprot No.：
P00750
种属：
Homo sapiens (Human)
样本类型：
serum, plasma, tissue homogenates
检测范围：
0.156 ng/ml-10 ng/ml
灵敏度：
0.039 ng/ml
反应时间：
1-5h
样本体积：
50-100ul
检测波长：
450 nm
研究领域：
Cardiovascular
测定原理：
quantitative
测定方法：
Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

　Intra-Assay PrecisionInter-Assay PrecisionSample123123n202020202020Mean(ng/ml)1.3541.2721.2161.1081.2411.297SD0.0340.0330.0260.0340.0430.046CV(%)3.7283.7673.0554.2454.9885.186

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human t-PA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

Sample

Serum(n=4)

1:1

Average %

Range %

84-92

1:2

Average %

Range %

90-98

1:4

Average %

Range %

95-104

1:8

Average %

102

Range %

96-108

回收率：

The recovery of human t-PA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	89	83-96
EDTA plasma (n=4)	100	95-105

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.


ng/ml	OD1	OD2	Average	Corrected
10	2.264	2.298	2.281	2.134
5	1.841	1.869	1.855	1.708
2.5	1.274	1.297	1.286	1.139
1.25	0.862	0.897	0.880	0.733
0.625	0.597	0.574	0.586	0.439
0.313	0.415	0.439	0.427	0.280
0.156	0.251	0.272	0.262	0.115
0	0.146	0.148	0.147