首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒

Human Elafin(PI3) ELISA kit

  • 中文名： 人弹性蛋白酶抑制剂(PI3)ELISA kit
货号: IPD-EL017952HU
Price： ￥2500
数量：
大包装询价

产品详情

  • 产品描述：
    人弹性蛋白酶抑制剂(PI3)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的PI3含量。PI3信号通路是一种细胞内信号转导途径，响应细胞外信号，调节多种细胞功能，如代谢、增殖、细胞存活、生长和血管生成等。其研究机制涉及PI3K（磷脂酰肌醇3-激酶）和AKT（蛋白激酶B）等关键基因，通过磷酸化下游靶标调控细胞生长、存活和凋亡等生命活动。试剂盒检测范围为7.8 pg/mL-500 pg/mL，灵敏度为1.95 pg/mL。该产品广泛应用于基础医学研究领域，如炎症相关信号通路解析、蛋白酶 - 抗蛋白酶系统平衡机制探索，以及疾病模型中 PI3 动态变化的定量分析，为呼吸系统疾病、免疫调节及组织损伤修复等方向的科研工作提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
  • 别名：
    Cementoin ELISA Kit; ELAF_HUMAN ELISA Kit; Elafin ELISA Kit; Elafin/Skalp ELISA Kit; Elastase Specific Inhibitor ELISA Kit; Elastase-specific inhibitor ELISA Kit; ES 1 ELISA Kit; ESI ELISA Kit; Peptidase inhibitor 3 ELISA Kit; Peptidase inhibitor 3; skin derived ELISA Kit; PI 3 ELISA Kit; PI-3 ELISA Kit; PI3 ELISA Kit; Pre elafin ELISA Kit; Protease inhibitor 3 skin derived ELISA Kit; Protease inhibitor 3; skin derived (SKALP) ELISA Kit; Protease inhibitor WAP3 ELISA Kit; SKALP ELISA Kit; Skin derived Anti leukoproteinase ELISA Kit; Skin-derived antileukoproteinase ELISA Kit; Trappin 2 ELISA Kit; WAP four disulfide core domain 14 ELISA Kit; WAP four disulfide core domain protein 14 ELISA Kit; WAP four-disulfide core domain protein 14 ELISA Kit; WAP3 ELISA Kit; WFDC14 ELISA Kit
  • 缩写：
    PI3
  • Uniprot No.：
    P19957
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    7.8 pg/mL-500 pg/mL
  • 灵敏度：
    1.95 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Signal Transduction
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：

    Intra-assay Precision (Precision within an assay): CV%<8%




    Three samples of known concentration were tested twenty times on one plate to assess.


    Inter-assay Precision (Precision between assays): CV%<10%




    Three samples of known concentration were tested in twenty assays to assess.



    　Intra-Assay PrecisionInter-Assay PrecisionSample123123n202020202020Mean(pg/ml)62.97463.99162.09061.96463.48261.586SD0.0420.0350.0370.0520.0470.050CV(%)5.3644.4254.7686.7105.9726.477







  • 线性度：

    To assess the linearity of the assay, samples were spiked with high concentrations of human Elafin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.


    Sample

    Serum(n=4)



    1:20

    Average %

    89



    Range %

    85-93



    1:40

    Average %

    100



    Range %

    95-105



    1:80

    Average %

    87



    Range %

    83-91



    1:160

    Average %

    105



    Range %

    101-109










  • 回收率：

    The recovery of human Elafin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range%



    Serum (n=5)

    86

    83-89



    EDTA plasma (n=4)

    99

    95-104










  • 标准曲线：

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

    pg/ml

    OD1

    OD2

    Average

    Corrected



    500

    2.783

    2.901

    2.842

    2.678



    250

    1.852

    1.798

    1.825

    1.661



    125

    1.210

    1.205

    1.208

    1.044



    62.5

    0.779

    0.789

    0.784

    0.620



    31.2

    0.483

    0.478

    0.481

    0.317



    15.6

    0.331

    0.354

    0.343

    0.179



    7.8

    0.279

    0.268

    0.274

    0.110



    0

    0.165

    0.163

    0.164












  • 货期：
    3-5 working days

客户数据及评论

折叠内容

大包装询价

×