首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠血小板因子4(PF-4/CXCL4)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的PF4含量。PF4即血小板因子4，是血小板α颗粒分泌的一种小分子趋化蛋白。其背景在于在止血、炎症和免疫反应等过程有重要作用。研究机制方面，PF4能与多种细胞表面受体相互作用，调节细胞功能，也参与调节血管生成、细胞增殖与迁移等生物学过程。试剂盒检测范围为3.125 ng/mL-200 ng/mL，灵敏度为1.603 ng/mL。该产品适用于心血管疾病模型、炎症机制研究、血小板功能分析等基础科研场景，例如在动脉粥样硬化、血栓性疾病或免疫相关动物模型中评估血小板活性及病理进程。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Pf4 ELISA Kit; Cxcl4 ELISA Kit; Scyb4 ELISA Kit; Platelet factor 4 ELISA Kit; PF-4 ELISA Kit; C-X-C motif chemokine 4 ELISA Kit
缩写：PF4
Uniprot No.：Q9Z126
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates
检测范围：3.125 ng/mL-200 ng/mL
灵敏度：1.603 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cardiovascular
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse PF-4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:200 | Average % | 86 | |||||
| Range % | 81-90 | ||||||
| 1:400 | Average % | 98 | |||||
| Range % | 92-104 | ||||||
| 1:800 | Average % | 97 | |||||
| Range % | 94-102 | ||||||
| 1:1600 | Average % | 94 | |||||
| Range % | 89-98 | ||||||
回收率：
| The recovery of mouse PF-4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 89 | 86-94 | |||||
| EDTA plasma (n=4) | 98 | 92-104 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| ng/mlOD1OD2AverageCorrected 2002.5012.4022.4522.308 1002.0931.9752.0341.890 501.3541.3831.3691.225 250.7780.7690.7740.630 12.50.3860.3710.3790.235 6.250.2680.2570.2630.119 3.1250.2090.2020.2060.062 00.1440.1430.144 | |||||||
本试剂盒所含材料：
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A micro ELISA plate ---The 96-well plate has been pre-coated with an anti-mouse PF4 antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
One vial Biotin-labeled PF4 antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the Biotin-antibody.
One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.
本试剂盒不含材料：
A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
An incubator can provide stable incubation conditions up to 37°C±5°C.
Centrifuge
Vortex
Squirt bottle, manifold dispenser, or automated microplate washer
Absorbent paper for blotting the microtiter plate
货期：3-5 working days
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